HMOX1 Antibody

Heme Oxygenase 1, Mouse Anti Human
Cat. No.
BT19621
Source
Synonyms
HO-1, HSP32, bK286B10, HMOX-1, Heme oxygenase 1, HMOX1, HO, HO1.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
HMOX1, also known as Heme Oxygenase-1, plays a crucial role in heme degradation. It catalyzes the breakdown of heme into biliverdin, iron, and carbon monoxide. This process is essential for recycling iron from senescent red blood cells and protecting cells from heme toxicity. HMOX1 is highly expressed in the spleen and is induced by various stimuli, including oxidative stress, inflammation, and hypoxia. Its upregulation has been linked to cytoprotective effects in various diseases, including cardiovascular disease, cancer, and neurodegenerative disorders. HMOX1's role in regulating inflammation and immune responses is complex and context-dependent. While it can suppress the production of pro-inflammatory cytokines in some settings, it can also promote inflammation in others.
Physical Appearance
Sterile filtered colorless solution.
Formulation
Supplied as a 1mg/ml solution in PBS, pH 7.4, containing 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For short-term storage (up to 1 month), store at 4°C. For long-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The product is stable for 12 months when stored at -20°C and for 1 month when stored at 4°C.
Applications
This HMOX1 antibody has been validated for use in ELISA, Western blot analysis, Flow cytometry, and ICC/IF assays. It exhibits high specificity and reactivity towards HMOX1. However, optimal working dilutions should be determined empirically for each application and experimental setup.
Synonyms
HO-1, HSP32, bK286B10, HMOX-1, Heme oxygenase 1, HMOX1, HO, HO1.
Purification Method
HMOX1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT1D6AT.
Immunogen
Anti-human HMOX1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HMOX1 amino acids 1-266 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and K light chain.

Product Science Overview

Importance of HO-1

HO-1 is highly inducible and is considered a key cytoprotective molecule against oxidative injury. The substrate, heme, is a potent pro-oxidant that can accelerate inflammation and cell death. Therefore, the activity of HO-1 is essential in mitigating these harmful effects . Research has shown that both humans and mice with defective HO-1 genes are highly susceptible to oxidative stress .

Anti-Heme Oxygenase 1 Antibody

The Mouse Anti-Human Heme Oxygenase 1 antibody is a monoclonal antibody that specifically targets the HO-1 protein in human samples. This antibody is widely used in various applications, including Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (Flow Cyt), and Enzyme-Linked Immunosorbent Assay (ELISA) .

  • Host Species: Mouse
  • Isotype: IgG1
  • Applications: WB, IHC, Flow Cyt, ELISA
  • Reactivity: Human, Mouse, Rat, Dog, Cow
Applications and Benefits

The Mouse Anti-Human HO-1 antibody is extensively validated and cited in numerous publications. It is used in research to study the role of HO-1 in various physiological and pathological processes, including its anti-inflammatory and cytoprotective functions .

  • Western Blot (WB): Used to detect HO-1 protein levels in different tissue samples.
  • Immunohistochemistry (IHC): Helps in visualizing the localization of HO-1 within tissue sections.
  • Flow Cytometry (Flow Cyt): Allows for the analysis of HO-1 expression in different cell populations.
  • ELISA: Used for quantifying HO-1 protein levels in various samples .

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Heme Oxygenase 1 Human Recombinant
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Source
Escherichia Coli.
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