HMGN3 Human

High Mobility Group Nucleosomal Binding Domain 3 Human Recombinant
Cat. No.
BT21391
Source
Escherichia Coli.
Synonyms
High Mobility Group Nucleosomal Binding Domain 3, TRIP7, TR-Interacting Protein 7, High Mobility Group Nucleosome-Binding Domain-Containing Protein 3, Thyroid Hormone Receptor Interacting Protein 7, Thyroid Receptor-Interacting Protein 7, Thyroid Hormone Receptor Interactor 7, PNAS-24, PNAS-25, TRIP-7, High mobility group nucleosome-binding domain-containing protein 3.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

HMGN3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 100 amino acids (1-77 a.a) and having a molecular mass of 10.8 kDa.
HMGN3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
High Mobility Group Nucleosomal Binding Domain 3 (HMGN3) binds to the thyroid hormone receptor beta, but only in the presence of thyroid hormone. Thyroid hormone receptors are hormone-dependent transcription factors that regulate the expression of specific target genes. HMGN3 is believed to decrease the compactness of the chromatin fiber in nucleosomes, thereby increasing transcription from chromatin templates.
Description
Recombinant human HMGN3, produced in E. coli, is a single, non-glycosylated polypeptide chain containing 100 amino acids (residues 1-77) with a molecular mass of 10.8 kDa. It includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The HMGN3 protein solution (0.5 mg/mL) is buffered with 20 mM Tris-HCl (pH 7.0) and contains 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity is determined to be greater than 95% by SDS-PAGE analysis.
Synonyms
High Mobility Group Nucleosomal Binding Domain 3, TRIP7, TR-Interacting Protein 7, High Mobility Group Nucleosome-Binding Domain-Containing Protein 3, Thyroid Hormone Receptor Interacting Protein 7, Thyroid Receptor-Interacting Protein 7, Thyroid Hormone Receptor Interactor 7, PNAS-24, PNAS-25, TRIP-7, High mobility group nucleosome-binding domain-containing protein 3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMPKRKSP ENTEGKDGSK VTKQEPTRRS ARLSAKPAPP KPEPKPRKTS AKKEPGAKIS RGAKGKKEEK QEAGKEGTEN.

Product Science Overview

Gene and Protein Structure

The HMGN3 gene is located on chromosome 6 at the position 6q14.1 . The gene produces multiple transcript variants encoding different isoforms of the HMGN3 protein . The protein itself is characterized by its ability to bind to nucleosomes, which are the fundamental units of chromatin. This binding is facilitated by the nucleosomal binding domain present in the protein.

Function and Mechanism

HMGN3 is known to interact with the thyroid hormone receptor beta in the presence of thyroid hormone . This interaction is significant because it influences the compactness of chromatin fibers. By reducing the compactness, HMGN3 enhances transcription from chromatin templates . This means that HMGN3 plays a vital role in regulating gene expression by modulating chromatin structure.

Additionally, HMGN3 has been implicated in the regulation of insulin and glucagon levels. It modulates the expression of pancreatic genes involved in insulin secretion . Specifically, HMGN3 regulates the expression of the glucose transporter SLC2A2 by binding to its promoter region and recruiting transcription factors such as PDX1 . This regulation is crucial for maintaining glucose homeostasis in the body.

Biological Significance

The biological significance of HMGN3 extends to various physiological processes. For instance, it is involved in the regulation of glycine concentration in synaptic junctions in the central nervous system by regulating the expression of the glycine transporter SLC6A9 . This function is essential for proper neurotransmission and synaptic function.

Moreover, HMGN3 may play a role in ocular development and astrocyte function . These diverse roles highlight the importance of HMGN3 in maintaining normal physiological functions across different tissues and organs.

Research and Clinical Implications

Research on HMGN3 has provided insights into its potential clinical implications. Given its role in regulating insulin secretion, HMGN3 could be a target for therapeutic interventions in diabetes and other metabolic disorders. Additionally, its involvement in chromatin remodeling and gene expression regulation makes it a potential target for cancer therapy, where dysregulation of these processes is often observed.

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