HMGA2 Human

High Mobility Group AT-Hook 2 Human Recombinant
Cat. No.
BT20980
Source
Escherichia Coli.
Synonyms
High Mobility Group AT-Hook 2, HMGIC, High-Mobility Group (Nonhistone Chromosomal) Protein Isoform I-C, High Mobility Group AT-Hook Protein, BABL, HMGI-C, LIPO, STQTL9, High Mobility Group Protein HMGI-C, HMGA2.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HMGA2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 117 amino acids (1-109 a.a) and having a molecular mass of 12.8kDa.
HMGA2 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The High Mobility Group AT-Hook 2 (HMGA2) protein belongs to the non-histone chromosomal high mobility group (HMG) family. HMG proteins act as architectural factors and are essential for enhancesome function. This protein possesses structural DNA-binding domains, suggesting a potential role as a transcriptional regulator. Notably, the identification of deletions, amplifications, and rearrangements of the HMGA2 gene in myxoid liposarcoma implicates its involvement in adipogenesis and mesenchymal differentiation. Studies involving the deletion of the corresponding gene in mice have shown that HMGA2 plays a role in diet-induced obesity. HMGA2 has been associated with conditions such as diffuse lipomatosis and 12q14 microdeletion syndrome.
Description
Recombinant HMGA2, of human origin, is produced in E.Coli. This non-glycosylated polypeptide chain comprises 117 amino acids (amino acids 1-109), resulting in a molecular weight of 12.8kDa. The HMGA2 protein is fused to an 8 amino acid His-tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The HMGA2 protein solution is provided at a concentration of 0.25mg/ml and contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol, 2mM DTT, and 250mM Imidazole.
Stability
For short-term storage (2-4 weeks), store the entire vial at 4°C. For extended storage, freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. It is crucial to avoid repeated freeze-thaw cycles.
Purity
SDS-PAGE analysis indicates a purity greater than 80.0%.
Synonyms
High Mobility Group AT-Hook 2, HMGIC, High-Mobility Group (Nonhistone Chromosomal) Protein Isoform I-C, High Mobility Group AT-Hook Protein, BABL, HMGI-C, LIPO, STQTL9, High Mobility Group Protein HMGI-C, HMGA2.
Source
Escherichia Coli.
Amino Acid Sequence
MSARGEGAGQ PSTSAQGQPA APAPQKRGRG RPRKQQQEPT GEPSPKRPRG RPKGSKNKSP SKAAQKKAEA TGEKRPRGRP RKWPQQVVQK KPAQEETEET SSQESAEEDL EHHHHHH.

Product Science Overview

Introduction

High Mobility Group AT-Hook 2 (HMGA2) is a small, non-histone chromosomal protein that plays a crucial role in various biological processes. It belongs to the High Mobility Group (HMG) family, which includes HMGA1a, HMGA1b, HMGA1c, and HMGA2 . HMGA2 is encoded by the HMGA2 gene and is known for its ability to bind to the minor groove of AT-rich DNA sequences through its three “AT-hook” DNA-binding motifs .

Structure and Function

HMGA2 consists of three “AT-hook” DNA-binding motifs and a negatively charged C-terminal motif . These motifs allow HMGA2 to bind to AT-rich regions of DNA, inducing DNA bending and facilitating the formation of higher-order chromatin structures . This binding is crucial for the regulation of gene expression and chromatin remodeling.

Biological Significance

HMGA2 is a multifunctional nuclear protein that is directly linked to several physiological and pathological processes, including:

  • Adipogenesis: HMGA2 plays a significant role in the differentiation of preadipocytes into adipocytes by regulating the expression of transcription factors such as Peroxisome proliferator-activated receptor γ (PPARγ) .
  • Stem Cell Maintenance: HMGA2 is essential for maintaining the stemness and renewal capacity of stem cells. It helps lock chromosomes into specific states, allowing human embryonic stem cells to retain their potency .
  • Tumorigenesis: HMGA2 is associated with increased cell proliferation and cell cycle dysregulation, leading to the development of various tumors . It is considered a potential therapeutic target for anticancer drugs .
Clinical Implications

Due to its involvement in critical biological processes, HMGA2 has been studied extensively for its potential therapeutic applications. It is considered a promising target for developing drugs to treat obesity and cancer . Efforts are ongoing to identify inhibitors that can specifically target HMGA2 and modulate its activity .

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