HMGA1 Human

High Mobility Group AT-Hook 1 Human Recombinant
Cat. No.
BT20897
Source
Escherichia Coli.
Synonyms
High mobility group protein HMG-I/HMG-Y, HMG-I(Y), High mobility group AT-hook protein 1, High mobility group protein A1, High mobility group protein R , HMGA1, HMGIY, HMG-R, HMGA1A.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HMGA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 115 amino acids (1-107 a.a.) and having a molecular mass of 12.7kDa (Molecular weight on SDS-PAGE will appear higher).
HMGA1 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The high mobility group protein HMG-I/HMG-Y, also known as HMGA1, is a member of the non-histone chromosomal high mobility group protein (HMG) family. This protein features a highly conserved AT-hook DNA-binding domain, which facilitates its binding to AT-rich sequences within the minor groove of chromosomal DNA. HMGA1 acts as an architectural chromatin-binding transcription factor, influencing DNA conformation by altering nuclear protein-DNA complexes. Moreover, HMGA1 plays a crucial role in various cellular processes, including the regulation of growth, the viral induction of the beta-IFN gene, and the control of inducible gene transcription.
Description
Recombinant HMGA1, of human origin, is produced in E. coli. This results in a single, non-glycosylated polypeptide chain composed of 115 amino acids (specifically, amino acids 1-107). The protein has a molecular mass of 12.7 kDa. However, the molecular weight observed on SDS-PAGE will be higher due to the presence of an 8 amino acid His-tag fused to the C-terminus of HMGA1. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The HMGA1 protein is supplied in a solution at a concentration of 0.25 mg/ml. The solution also contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 50% glycerol, and 0.2M NaCl.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For longer storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, the addition of a carrier protein (either 0.1% HSA or BSA) is advised. It is crucial to minimize the number of freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the HMGA1 protein is determined to be greater than 90.0% using SDS-PAGE analysis.
Synonyms
High mobility group protein HMG-I/HMG-Y, HMG-I(Y), High mobility group AT-hook protein 1, High mobility group protein A1, High mobility group protein R , HMGA1, HMGIY, HMG-R, HMGA1A.
Source
Escherichia Coli.
Amino Acid Sequence
MSESSSKSSQ PLASKQEKDG TEKRGRGRPR KQPPVSPGTA LVGSQKEPSE VPTPKRPRGR PKGSKNKGAA KTRKTTTTPG RKPRGRPKKL EKEEEEGISQ ESSEEEQLEH HHHHH.

Product Science Overview

Structure and Function

HMGA1 contains an AT-hook motif, which is a DNA-binding motif first described in the high-mobility group of non-histone chromosomal proteins HMGA1/2 . The AT-hook motif binds to AT-rich DNA sequences in the minor groove of B-DNA in a non-sequence specific manner . This binding property allows HMGA1 to interact with various DNA regions and influence gene expression.

Role in Disease

HMGA1 has been implicated in several diseases, particularly in cancer. It has been demonstrated to modulate cellular proliferation, invasion, and apoptosis, often associated with a poor prognosis in various carcinomas . Additionally, HMGA1 is an important positive regulator of hepatitis B virus (HBV) transcription. It binds to a conserved ATTGG site within the enhancer II/core promoter (EII/Cp) and recruits transcription factors, enhancing viral gene expression and genome replication .

Recombinant HMGA1

Recombinant HMGA1 refers to the artificially synthesized version of the protein, produced using recombinant DNA technology. This technology involves inserting the HMGA1 gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. Recombinant HMGA1 is used in various research applications to study its structure, function, and interactions with other molecules.

Research and Applications

Research on HMGA1 has provided valuable insights into its role in gene regulation and disease. For example, studies have shown that HMGA1-mediated upregulation of HBV transcription involves the HBV X protein (HBx) interacting with the SP1 transcription factor to activate the HMGA1 promoter . Additionally, targeting endogenous HMGA1 through RNA interference has been shown to facilitate HBV clearance in a mouse model of HBV persistence .

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