HLA-F Human

Major Histocompatibility Complex Class I F Human Recombinant
Cat. No.
BT5436
Source
Escherichia Coli.
Synonyms
CDA12, HLA-5.4, HLA-CDA12, HLAF, HLA class I histocompatibility antigen, alpha chain F, HLA F antigen, Leukocyte antigen F, MHC class I antigen F, HLA-F.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HLA-F Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 307 amino acids (22-305a.a) and having a molecular mass of 35.1kDa.
HLA-F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The major histocompatibility complex (MHC) class I F molecule, also known as HLA-F, plays a crucial role in the immune system's ability to recognize and respond to foreign invaders. As part of the MHC family, HLA-F participates in antigen presentation, a process where cells display fragments of proteins, or peptides, on their surface for inspection by T cells, the immune system's sentinels. Belonging to the class I molecules, HLA-F is expressed on most cell types and presents peptides derived from within the cell, providing a snapshot of the cell's internal environment to the immune system.
Description
Recombinant HLA-F, produced in E. coli, is a single polypeptide chain devoid of glycosylation. It comprises 307 amino acids (specifically, residues 22 to 305), resulting in a molecular weight of 35.1 kDa. For purification and detection purposes, the HLA-F protein is engineered with a 23-amino acid His-tag attached to its N-terminus. Proprietary chromatographic techniques ensure the protein's isolation and purification.
Physical Appearance
The product appears as a clear, colorless solution that has undergone sterile filtration.
Formulation
The HLA-F protein is supplied in a solution containing 0.25 mg/ml of the protein in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. To further enhance stability during long-term storage, adding a carrier protein like HSA or BSA (0.1%) is advisable. Repeated freezing and thawing cycles should be avoided to maintain product integrity.
Purity
The purity of HLA-F is determined using SDS-PAGE analysis, and it consistently exceeds 90%.
Synonyms
CDA12, HLA-5.4, HLA-CDA12, HLAF, HLA class I histocompatibility antigen, alpha chain F, HLA F antigen, Leukocyte antigen F, MHC class I antigen F, HLA-F.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSGSHSLRY FSTAVSRPGR GEPRYIAVEY VDDTQFLRFD SDAAIPRMEP REPWVEQEGP QYWEWTTGYA KANAQTDRVA LRNLLRRYNQ SEAGSHTLQG MNGCDMGPDG RLLRGYHQHA YDGKDYISLN EDLRSWTAAD TVAQITQRFY EAEEYAEEFR TYLEGECLEL LRRYLENGKE TLQRADPPKA HVAHHPISDH EATLRCWALG FYPAEITLTW QRDGEEQTQD TELVETRPAG DGTFQKWAAV VVPPGEEQRY TCHVQHEGLP QPLILRWEQS PQPTIPI.

Product Science Overview

Introduction

The Major Histocompatibility Complex (MHC) is a set of genes that play a crucial role in the immune system by presenting peptide fragments to T cells. These genes are highly polymorphic and are essential for the adaptive immune response. MHC Class I molecules are one of the two primary classes of MHC molecules, the other being MHC Class II .

Structure and Function

MHC Class I molecules are heterodimers consisting of two polypeptide chains: an alpha chain and a beta-2 microglobulin (B2M) chain . These molecules are expressed on the surface of almost all nucleated cells and are responsible for presenting endogenous peptides, typically derived from cytosolic proteins, to cytotoxic T lymphocytes (CTLs) . This presentation is crucial for the immune system to recognize and eliminate infected or cancerous cells .

MHC Class I F

MHC Class I F (HLA-F in humans) is a non-classical MHC Class I molecule. Unlike classical MHC Class I molecules (HLA-A, HLA-B, and HLA-C), which are highly polymorphic and ubiquitously expressed, HLA-F has limited polymorphism and a more restricted expression pattern . HLA-F is primarily expressed in the placenta and certain immune cells, and it plays a role in immune regulation and tolerance .

Recombinant HLA-F

Recombinant HLA-F refers to the laboratory-produced version of the HLA-F protein. This recombinant protein is used in various research applications to study its structure, function, and interactions with other molecules. The production of recombinant HLA-F involves cloning the HLA-F gene into an expression vector, which is then introduced into a host cell line to produce the protein .

Applications

Recombinant HLA-F is used in several research areas, including:

  • Immunology: To study the role of HLA-F in immune regulation and its interactions with other immune cells.
  • Transplantation: To understand the role of HLA-F in transplant tolerance and rejection.
  • Cancer Research: To investigate the potential role of HLA-F in tumor immune evasion and its potential as a therapeutic target .

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