HK1 Antibody

Hexokinase-1, Mouse Anti Human
Cat. No.
BT19148
Source
Synonyms
Hexokinase-1, EC 2.7.1.1, Hexokinase type I, HK I, Brain form hexokinase, HK1-ta, HK1-tb, HXK1, HK1.
Appearance
Sterile Filtered clear solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Hexokinases are enzymes that play a crucial role in glucose metabolism by phosphorylating glucose to glucose-6-phosphate, thereby committing it to the glycolytic pathway. Hexokinase 1, encoded by the HXK1 gene, is a ubiquitous form of hexokinase found localized to the outer mitochondrial membrane. Genetic mutations in HXK1 have been linked to hemolytic anemia caused by hexokinase deficiency. Alternative splicing of HXK1 results in five distinct transcript variants, each encoding a different isoform, with some exhibiting tissue specificity. Notably, all isoforms share an identical protein sequence except for their unique N-termini. A sixth transcript variant has also been identified; however, the presence of multiple stop codons suggests it may not encode a functional protein.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The antibody is supplied as a 1 mg/ml solution in phosphate-buffered saline (PBS) at pH 7.4 with 0.1% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), the antibody should be kept at 4°C. For extended storage, it is recommended to store at -20°C. To maintain stability, avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody has a shelf life of 12 months when stored at -20°C and 1 month when stored at 4°C.
Applications
The Hexokinase-1 antibody has undergone rigorous testing using ELISA and Western blot analysis to confirm its specificity and reactivity. However, it's important to note that optimal working dilutions may vary depending on the specific application. For Western blot analysis, a dilution range of 1:1,000 to 1:2,000 is recommended, with a starting dilution of 1:1,000.
Synonyms
Hexokinase-1, EC 2.7.1.1, Hexokinase type I, HK I, Brain form hexokinase, HK1-ta, HK1-tb, HXK1, HK1.
Purification Method
Hexokinase-1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P4D7AT.
Immunogen
Anti-human Hexokinase-1 mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Hexokinase-1 amino acids 1-917 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and κ light chain.

Product Science Overview

Hexokinase Isoforms

There are four distinct isoforms of hexokinase in mammals:

  1. Hexokinase I: Predominantly found in the brain and is associated with the outer mitochondrial membrane.
  2. Hexokinase II: Found in insulin-sensitive tissues such as skeletal muscle and adipose tissue.
  3. Hexokinase III: Less well understood but is also associated with the outer mitochondrial membrane.
  4. Hexokinase IV (Glucokinase): Found in the liver and pancreatic beta cells, playing a key role in glucose homeostasis.
Hexokinase-1 in Cancer

Hexokinase-1 is particularly important in cancer cells, where it is involved in the Warburg effect. This phenomenon describes how cancer cells preferentially utilize glycolysis for energy production, even in the presence of oxygen. This allows for rapid cell growth and proliferation, as glycolysis provides both energy and biosynthetic precursors necessary for cell division .

Mouse Anti-Human Hexokinase-1 Antibody

The Mouse Anti-Human Hexokinase-1 Antibody is a monoclonal antibody produced by immunizing mice with a synthetic peptide corresponding to the sequence of human hexokinase-1. This antibody is highly specific and is used in various research applications, including:

  • Western Blotting: To detect the presence of hexokinase-1 in cell extracts.
  • Immunohistochemistry: To visualize the localization of hexokinase-1 in tissue sections.
  • Immunofluorescence: To study the distribution of hexokinase-1 within cells.
Applications and Importance

The Mouse Anti-Human Hexokinase-1 Antibody is a valuable tool in biomedical research. It helps scientists understand the role of hexokinase-1 in various physiological and pathological processes, including:

  • Cancer Research: Investigating the role of hexokinase-1 in tumor metabolism and growth.
  • Metabolic Studies: Understanding how hexokinase-1 contributes to glucose metabolism in different tissues.
  • Neuroscience: Studying the function of hexokinase-1 in the brain and its involvement in neurodegenerative diseases.

In summary, hexokinase-1 is a critical enzyme in glucose metabolism, and the Mouse Anti-Human Hexokinase-1 Antibody is an essential tool for researchers studying its function and regulation in health and disease .

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