HIV-2 gp36, 17kDa

HIV-2 gp36 Recombinant
Cat. No.
BT24425
Source
Escherichia Coli.
Synonyms
Appearance

Sterile Filtered clear solution.

Purity

Protein is >90% pure as determined by 10% PAGE (coomassie staining).

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Recombinant HIV-2 gp36 produced in E.coli is a non-glycosylated polypeptide chain contains HIV2 subtype A gp 36 sequence and purified by proprietary chromatographic technique.

Product Specs

Introduction
Human immunodeficiency viruses 1 and 2 (HIV-1 and HIV-2) exhibit distinct RNA packaging mechanisms. HIV-1 demonstrates non-selective RNA binding, while HIV-2 preferentially packages its own Gag protein mRNA. This difference contributes to the higher mutation rate observed in HIV-1. Transmission routes for HIV-2 are similar to HIV-1, involving exposure to infected bodily fluids such as blood, semen, vaginal secretions, and tears. However, HIV-2 infection progresses at a slower rate, with immunodeficiency developing more gradually compared to HIV-1. In the initial stages, HIV-2 exhibits lower infectivity than HIV-1, but this infectivity increases as the disease advances. Significant distinctions between the two viruses encompass reduced pathogenicity of HIV-2, enhanced immune system control over HIV-2 infection, and a degree of independence from CD4 receptors in HIV-2. Despite substantial differences in sequence and phenotype, the envelope proteins of HIV-1 and HIV-2 share structural similarities. Both membrane-anchored proteins assemble into six-helix bundles from their N-terminal and C-terminal ectodomain regions, a characteristic observed in various viral and cellular fusion proteins, which appears to drive the fusion process. While HIV-1 gp41 helical regions exhibit higher stability in forming six-helix bundles compared to HIV-2 gp41, HIV-2 fusion occurs at a lower temperature threshold (25 °C), does not necessitate calcium ions (Ca2+) in the surrounding environment, remains unaffected by cytochalasin B treatment on target cells, and demonstrates insensitivity to variations in glycosphingolipid composition within target cell membranes.
Description
Recombinant HIV-2 gp36, a non-glycosylated polypeptide, is produced in E. coli. This protein corresponds to the HIV-2 subtype A gp36 sequence and is purified using a proprietary chromatographic method.
Physical Appearance

Sterile Filtered clear solution.

Formulation
The formulation for HIV-2 gp36 consists of phosphate-buffered saline (PBS), 25mM potassium carbonate (K2CO3), and 0.025% sodium azide (NaN3).
Stability
For short-term storage (up to 2-4 weeks), store the intact vial at 4 °C. For extended storage, freeze at -20 °C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 90% as determined by SDS-PAGE analysis (10% gel, Coomassie staining).
Applications
This product is suitable for applications such as enzyme-linked immunosorbent assay (ELISA) and lateral flow assays.
Source
Escherichia Coli.

Product Science Overview

Introduction

Human Immunodeficiency Virus type 2 (HIV-2) is a retrovirus that causes a form of AIDS similar to that caused by HIV-1, but with a slower progression. One of the key proteins in the HIV-2 virus is gp36, a transmembrane envelope protein comparable to gp41 in HIV-1 .

Structure and Function

The gp36 protein is an essential component of the HIV-2 envelope. It plays a crucial role in the virus’s ability to infect host cells. The protein is involved in the fusion of the viral membrane with the host cell membrane, a critical step for viral entry and infection . The recombinant form of gp36 is a non-glycosylated polypeptide chain, typically expressed in Escherichia coli (E. coli) for research and diagnostic purposes .

Expression and Purification

Recombinant HIV-2 gp36 is produced using E. coli expression systems. The gene encoding the gp36 protein is synthesized with codons optimized for E. coli expression. This recombinant protein is then purified using proprietary chromatographic techniques to achieve high purity levels, often exceeding 95% . The purified protein is used in various applications, including enzyme-linked immunosorbent assays (ELISA) and Western blotting (WB) .

Applications

Recombinant HIV-2 gp36 is widely used in research and diagnostic assays. It is immunoreactive with sera from HIV-2 infected individuals, making it a valuable tool for detecting HIV-2 specific antibodies . This specificity is crucial for distinguishing between infections caused by HIV-1 and HIV-2, which is important for accurate diagnosis and treatment planning .

Importance in Research

The study of recombinant HIV-2 gp36 provides insights into the mechanisms of viral entry and infection. By understanding how gp36 interacts with host cells, researchers can develop targeted therapies and vaccines to combat HIV-2. Additionally, the use of recombinant proteins in diagnostic assays enhances the accuracy and reliability of HIV testing .

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