HIV-2 gp32, Biotin
HIV-2 gp32 Recombinant, Biotin Labeled
Cat. No.
BT24101
Source
Escherichia Coli.
Synonyms
Appearance
Sterile filtered colorless clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
HIV-2 gp32 Biotin Labeled recombinant- contains the full-length sequence of HIV-2 envelope immunodominant regions gp32 having a Mw of 32kDa and fused to a beta-galactosidase at N-terminus.
Product Specs
Introduction
Human Immunodeficiency Viruses 1 and 2 (HIV-1 and HIV-2) exhibit different RNA packaging mechanisms. HIV-1 can bind to a wider range of RNA molecules, while HIV-2 preferentially binds to its own Gag protein mRNA. This difference contributes to the higher mutation rate observed in HIV-1. Transmission routes for both viruses are identical, primarily occurring through exposure to infected bodily fluids like blood, semen, vaginal fluids, and tears. Notably, HIV-2 infection is characterized by a slower progression to immunodeficiency, reduced infectivity in early stages compared to HIV-1, and increased infectivity as the disease progresses. Significant distinctions include HIV-2's lower pathogenicity compared to HIV-1, enhanced immune system control over HIV-2, and a degree of independence from CD4 cells. Despite variations in sequence and phenotype, the envelope proteins of HIV-1 and HIV-2 share structural similarities. Both membrane-anchored proteins assemble into 6-helix bundles from their N-terminal and C-terminal ectodomain regions, a characteristic common to various viral and cellular fusion proteins, which is believed to drive membrane fusion. While HIV-1 gp41 helical regions can form more stable 6-helix bundles compared to those of HIV-2 gp41, HIV-2 fusion occurs at a lower temperature (25°C), does not require extracellular calcium ions, remains unaffected by cytochalasin B treatment on target cells, and shows insensitivity to the glycosphingolipid composition of target membranes.
Description
The product is a biotin-labeled recombinant form of the HIV-2 gp32 protein. It encompasses the complete amino acid sequence of the immunodominant regions of the HIV-2 envelope protein gp32, resulting in a molecular weight of 32 kDa. The recombinant gp32 is fused to a beta-galactosidase enzyme at its N-terminus.
Physical Appearance
The product appears as a clear solution without any color and has been sterilized by filtration.
Formulation
The product is supplied in a solution containing 0.01 M sodium carbonate (Na2CO3), 10 mM ethylenediaminetetraacetic acid (EDTA), 14 mM beta-mercaptoethanol, and 0.02% Sarcosyl.
Purity
The purity of HIV-2 gp32 is determined to be greater than 95% using SDS-PAGE analysis.
Stability
While HIV-2 gp-32 maintains stability at room temperature for a period of 3 weeks, it is recommended to store the product at a temperature of 4°C to ensure optimal long-term stability.
Source
Escherichia Coli.
Product Science Overview
Introduction
The HIV-2 gp32 Recombinant, Biotin Labeled protein is a significant tool in the study of HIV-2, a less common but equally important variant of the Human Immunodeficiency Virus (HIV). This recombinant protein is engineered to include the full-length sequence of the HIV-2 envelope immunodominant region, gp32, and is fused to a beta-galactosidase at the N-terminus .
HIV-2 and Its Differences from HIV-1
HIV-2 is less prevalent than HIV-1 and is primarily found in West Africa. Despite their similarities, there are notable differences between the two viruses:
- Pathogenicity: HIV-2 is generally less pathogenic than HIV-1, meaning it causes disease more slowly and less severely .
- Transmission: Both viruses are transmitted through exposure to bodily fluids, but HIV-2 is less infectious in the early stages .
- Immune Response: HIV-2 often exhibits enhanced immune control compared to HIV-1 .
Structure and Function of gp32
Recombinant Protein and Biotin Labeling
Applications in Research
The biotin-labeled recombinant HIV-2 gp32 protein is used in a variety of research applications, including:
- Vaccine Development: Studying the immune response to gp32 can help in the development of vaccines against HIV-2.
- Diagnostic Tools: The protein can be used in assays to detect the presence of HIV-2 in biological samples.
- Therapeutic Research: Understanding the structure and function of gp32 can aid in the development of antiviral drugs targeting HIV-2 .
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
