HIV-2 gp32 is a recombinant protein derived from the Human Immunodeficiency Virus type 2 (HIV-2). This protein is a part of the envelope glycoprotein complex of the virus, which plays a crucial role in the virus’s ability to infect host cells. The recombinant form of HIV-2 gp32 is produced using genetic engineering techniques, typically involving the expression of the protein in a host organism such as Escherichia coli .
The HIV-2 gp32 recombinant protein contains the full-length sequence of the immunodominant regions of the HIV-2 envelope glycoprotein gp32. It is often fused with beta-galactosidase at the N-terminus, resulting in a protein with a molecular weight of approximately 114 kDa . The protein is typically formulated in a sterile, filtered, colorless, clear solution containing various stabilizing agents .
The gp32 protein is a critical component of the HIV-2 envelope, which facilitates the virus’s entry into host cells. It is involved in the fusion of the viral membrane with the host cell membrane, a process essential for the virus to deliver its genetic material into the host cell . The recombinant form of gp32 retains the immunodominant regions of the native protein, making it useful for various research and diagnostic applications.
HIV-2 gp32 recombinant protein is widely used in research and diagnostic settings. It is particularly valuable in enzyme-linked immunosorbent assays (ELISA) and Western blot assays for the detection of HIV-2 antibodies in patient sera . The protein’s immunoreactivity with sera from HIV-2 infected individuals makes it an excellent antigen for early detection of HIV seroconverters with minimal specificity problems .
The recombinant HIV-2 gp32 protein is produced in Escherichia coli and purified to a high degree of purity, typically greater than 95% as determined by SDS-PAGE . The protein is formulated in a solution containing sodium carbonate, sodium EDTA, beta-mercaptoethanol, and sarcosyl to ensure stability and maintain its functional integrity .