HIV-1 TAT Clade-A
Sterile Filtered and lyophilized, though might appear as a solution as a result of the glycerol content.
Description
HIV-1 TAT Clade-A Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain having the Accession number: AAL06113.1
Product Specs
HIV-1 TAT Clade-A Recombinant, produced in E.coli, is a single, non-glycosylated polypeptide chain with Accession number AAL06113.1.
While sterile filtered and lyophilized, it may appear as a solution due to the glycerol content.
For short-term storage (2-4 weeks), store at 4°C. For extended storage, freeze at -20°C. Minimize repeated freeze-thaw cycles.
Product Science Overview
The Human Immunodeficiency Virus type 1 (HIV-1) is a highly recombinogenic virus, meaning it has a strong tendency to undergo genetic recombination. This characteristic allows the virus to evolve rapidly and adapt to various selective pressures, such as the host immune response and antiretroviral therapies . One of the key proteins involved in HIV-1’s lifecycle is the Transactivator of Transcription (Tat) protein. The Tat protein is crucial for the efficient transcription of the HIV-1 genome and plays a significant role in the virus’s replication and pathogenesis .
The HIV-1 Tat protein is a 14-kDa protein encoded by the tat gene. It is one of the first proteins expressed after HIV-1 infection and is essential for the initiation and elongation of HIV-1 transcription . Unlike typical transcription factors that bind to DNA, Tat is an RNA-binding protein that recognizes a specific sequence known as the Transactivator Response Element (TAR) on the HIV-1 RNA molecule . Tat recruits the host positive transcription elongation factor b (p-TEFb) to the TAR RNA structure, which in turn phosphorylates the C-terminal domain of RNA polymerase II, thereby enhancing transcription elongation .
HIV-1 is classified into different clades or subtypes based on genetic variations. Clade A is one of the major subtypes and is predominantly found in West and Central Africa . The genetic diversity among different clades of HIV-1 can influence the virus’s transmission, pathogenesis, and response to antiretroviral therapies. Recombinant forms of HIV-1, which result from the recombination of different clades, are also common and contribute to the virus’s genetic diversity .
Recombinant forms of HIV-1 arise when a single cell is infected with two or more different viral strains, leading to the generation of recombinant viruses during reverse transcription . These recombinant viruses can have unique genetic and phenotypic properties, which may affect their transmissibility, virulence, and resistance to antiretroviral drugs . In regions where multiple HIV-1 clades co-circulate, such as West and Central Africa, recombinant forms are particularly common .
The HIV-1 Tat Clade-A Recombinant refers to a recombinant form of HIV-1 that includes the Tat protein from Clade A. This recombinant form can have unique properties that distinguish it from other clades and recombinant forms. The Tat protein from Clade A may have specific amino acid sequences and structural features that influence its function and interactions with host cellular factors. Understanding the properties of HIV-1 Tat Clade-A Recombinant is important for developing targeted therapies and vaccines.
