HIV-1 gp41 Long, HRP
Description
Product Specs
Product Science Overview
Human Immunodeficiency Virus type 1 (HIV-1) is a retrovirus that primarily targets the immune system, leading to a progressive failure of the immune system and increased susceptibility to opportunistic infections. HIV-1 primarily infects vital cells in the human immune system, such as helper T cells (specifically CD4+ T cells), macrophages, and dendritic cells .
The gp41 protein is a transmembrane glycoprotein that plays a crucial role in the fusion of the viral membrane with the host cell membrane, facilitating the entry of the virus into the host cell. It is part of the envelope glycoprotein complex (Env) of HIV-1, which also includes gp120 .
Recombinant proteins are proteins that are genetically engineered in the laboratory by inserting the gene encoding the protein into an expression system, such as bacteria (e.g., Escherichia coli). These proteins are then produced in large quantities for various research and diagnostic purposes .
Horseradish Peroxidase (HRP) is an enzyme commonly used in biochemistry applications, particularly in immunoassays. HRP labeling involves attaching the HRP enzyme to a protein or antibody, allowing for the detection of the protein or antibody through a colorimetric reaction .
The HIV-1 gp41 Long Recombinant, HRP Labeled protein is a recombinant protein derived from Escherichia coli. It contains immunodominant regions from the HIV-1 gp41 protein and is fused to beta-galactosidase at the N-terminus . This fusion protein is then labeled with HRP, allowing for its use in various immunoassays to detect HIV-1 antibodies in serum samples .
Key Characteristics:
- Source: Escherichia coli
- Physical Appearance: Sterile filtered colorless clear solution
- Formulation: 8M urea, 20mM Tris-HCl pH 8.0, 10mM beta-mercaptoethanol
- Purity: Greater than 95.0% as determined by SDS-PAGE
- Stability: Stable at 4°C for 1 week; should be stored below -18°C to prevent freeze-thaw cycles
- Specificity: Immunoreactive with HIV positive serum
The HIV-1 gp41 Long Recombinant, HRP Labeled protein is primarily used in laboratory research for the detection of HIV-1 antibodies. It is utilized in various immunoassays, such as ELISA (Enzyme-Linked Immunosorbent Assay), to identify the presence of antibodies against HIV-1 in serum samples. This is crucial for the diagnosis and monitoring of HIV-1 infection .
