HINT1 Human
Histidine Triad Nucleotide Binding Protein 1 Human Recombinant
Cat. No.
BT21460
Source
Escherichia Coli.
Synonyms
HINT, PKCI-1, PRKCNH1, FLJ30414, FLJ32340, HINT1, Histidine triad nucleotide-binding protein 1, Adenosine 5'-monophosphoramidase, Protein kinase C inhibitor 1, Protein kinase C-interacting protein 1, PKCI1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
HINT1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 126 amino acids (1-126 a.a.) and having a molecular mass of 13.8 kDa.
The HINT1 is purified by proprietary chromatographic techniques.
The HINT1 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
HINT1, alternatively known as Histidine triad nucleotide-binding protein 1, belongs to a protein superfamily characterized by a near C-terminal HXHXHXX motif (H=Histidine, X=a hydrophobic amino acid). This motif interacts with the ?-phosphate of nucleotide substrates. HINT1 exhibits hydrolytic activity towards adenosine 5'-monophosphoramidate substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. Initially, it was thought to inhibit protein kinase C and function as a haplod-insufficient tumor suppressor, with observations of spontaneous tumor development in Hint+/- and Hint-/- models. However, its precise physiological role remains to be fully elucidated.
Description
Recombinant human HINT1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 126 amino acids (1-126 a.a.). It has a molecular weight of 13.8 kDa. The purification of HINT1 is achieved through proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The HINT1 solution is supplied in 20mM Tris buffer at pH 8.0 with 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during storage, adding a carrier protein (0.1% HSA or BSA) is advised. It is crucial to avoid repeated freeze-thaw cycles.
Purity
The purity of HINT1 is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
HINT, PKCI-1, PRKCNH1, FLJ30414, FLJ32340, HINT1, Histidine triad nucleotide-binding protein 1, Adenosine 5'-monophosphoramidase, Protein kinase C inhibitor 1, Protein kinase C-interacting protein 1, PKCI1.
Source
Escherichia Coli.
Amino Acid Sequence
MADEIAKAQV ARPGGDTIFG KIIRKEIPAK IIFEDDRCLA FHDISPQAPT HFLVIPKKHI SQISVAEDDD ESLLGHLMIV GKKCAADLGL NKGYRMVVNE GSDGGQSVYH VHLHVLGGRQ MHWPPG.
Product Science Overview
Introduction
Histidine Triad Nucleotide Binding Protein 1 (HINT1) is a highly conserved protein that belongs to the histidine triad superfamily. This family is characterized by the presence of a histidine triad motif (His-X-His-X-His-X, where X is a hydrophobic amino acid) which is crucial for its function . HINT1 is involved in various biological processes and has been studied for its potential therapeutic applications.
Structure and Function
HINT1 is a small protein with a molecular mass of approximately 13.8 kDa . It hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2 . The protein interacts with these substrates via its histidine triad motif, which is essential for its enzymatic activity .
Biological Roles
HINT1 has been implicated in several biological pathways and processes:
- Tumor Suppression: HINT1 is considered a tumor suppressor gene. Loss of HINT1 function increases susceptibility to carcinogenesis in mice .
- Neurological Functions: HINT1 plays important roles in diverse neuropsychiatric diseases, including schizophrenia, mood disorders, drug addiction, and inherited peripheral neuropathies .
- Liver Fibrosis: Recombinant human HINT1 (rhHint1) has been shown to attenuate liver fibrosis induced by carbon tetrachloride (CCl4) in rats. This effect is achieved by inhibiting the TGF-β/Smad and Wnt/β-catenin signaling pathways .
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