HEV ORF2 (403-461 a.a.)
Description
Product Specs
Product Science Overview
Hepatitis E virus (HEV) is a zoonotic pathogen responsible for causing hepatitis in both humans and animals. It is a significant cause of acute hepatitis outbreaks worldwide. HEV is a single-stranded, positive-sense RNA virus with a genome length of approximately 7.2 kb. The genome encodes three well-defined open reading frames (ORFs): ORF1, ORF2, and ORF3 .
The recombinant ORF2 protein fragment, spanning amino acids 403 to 461, is a specific segment of the ORF2 protein. This fragment has been expressed in various systems, including Escherichia coli, to facilitate research and diagnostic applications. The recombinant protein is often used in enzyme-linked immunosorbent assays (ELISA) and Western blot (WB) techniques due to its high purity and reactivity with HEV-positive serum .
The recombinant ORF2 (403-461 a.a.) protein is typically expressed in E. coli, a common host for producing recombinant proteins. The expression system ensures high yields and allows for the purification of the protein to greater than 95% purity. The purified protein is tag-free, making it suitable for various applications without interference from fusion tags .
The recombinant ORF2 (403-461 a.a.) protein is widely used in research and diagnostic settings. Its primary applications include:
- ELISA: The protein is used as an antigen in ELISA to detect antibodies against HEV in serum samples.
- Western Blot: It serves as a target protein in Western blot assays to identify HEV-specific antibodies.
- Vaccine Development: The ORF2 protein is a potential candidate for developing vaccines against HEV due to its immunogenic properties .
