HES2 Human
Hairy and Enhancer of Split 2 Human Recombinant
Cat. No.
BT13522
Source
Escherichia Coli.
Synonyms
bHLHb40, hairy and enhancer of split 2, Class B basic helix-loop-helix protein 40, HES2.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
HES2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 196 amino acids (1-173 a.a.) and having a molecular mass of 20.9kDa.
HES2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
HES2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Hairy and enhancer of split 2 (HES2), a member of the HES family, contains a basic helix-loop-helix (bHLH) domain and an orange domain. HES family proteins interact with TLE, the mammalian homologue of Groucho, through the carboxy-terminal WRPW motif. Notch signaling activation triggers HES family gene activation via the interaction between the Notch intracellular domain and RBPSUH (CSL). HES2 expression is observed in the placenta, pancreatic cancer, colon cancer with RER, cervical cancer, and head and neck tumors.
Description
Recombinant human HES2, produced in E. coli, is a single, non-glycosylated polypeptide chain of 196 amino acids (residues 1-173) with a molecular weight of 20.9 kDa. It includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
HES2 protein solution (1 mg/ml) is supplied in 20 mM Tris-HCl buffer (pH 8.0), containing 0.4 M urea and 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 80.0% by SDS-PAGE analysis.
Synonyms
bHLHb40, hairy and enhancer of split 2, Class B basic helix-loop-helix protein 40, HES2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGLPRRA GDAAELRKSL KPLLEKRRRA RINQSLSQLK GLILPLLGRE NSNCSKLEKA DVLEMTVRFL QELPASSWPT AAPLPCDSYR EGYSACVARL ARVLPACRVL EPAVSARLLE HLWRRAASAT LDGGRAGDSS GPSAPAPAPA SAPEPASAPV PSPPSPPCGP GLWRPW.
Product Science Overview
Gene and Protein Structure
Expression and Function
HEY2 is expressed in various tissues during embryonic development and in adults. It is particularly significant in the cardiovascular system, where it regulates arterial-venous cell fate decisions . The gene is expressed in the lateral plate mesoderm before vessel formation and continues to be expressed in the aorta but not in veins .
Role in Development and Disease
HEY2 plays a critical role in:
- Cardiovascular Development: It is involved in the formation of arteries and veins, influencing the arterial endothelial identity even before the onset of blood flow .
- Bone Remodeling: In mice, HEY2 regulates bone mass by influencing osteoblast activity and bone resorption. Overexpression of HEY2 in osteoblasts leads to decreased bone mass, while its inactivation increases bone mass .
Recombinant HEY2
Recombinant human HEY2 is produced using recombinant DNA technology, which involves inserting the HEY2 gene into a suitable expression system, such as bacteria or mammalian cells, to produce the protein in large quantities. This recombinant protein can be used in various research applications, including studies on gene regulation, developmental biology, and disease mechanisms .
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