HSV-8 M

Herpes Simplex Virus-8 Mosaic Recombinant
Cat. No.
BT19235
Source
Synonyms
Appearance
Sterile Filtered clear solution.
Purity
HSV-8 Mosaic protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.Coli derived recombinant protein contains the C-terminal immunodominant regions from ORF65 140-170 a.a. and N-terminal regions from ORF8 32-62 a.a. The protein is fused with a GST tag.

Product Specs

Introduction
Herpes simplex virus (HSV) entry into host cells is mediated by interactions between viral glycoproteins and cell surface receptors. The virus particle's envelope, upon binding to specific cell surface receptors, fuses with the cell membrane. This fusion creates a pore, facilitating viral entry into the host cell. HSV entry unfolds in a sequential manner, mirroring other viruses. Initially, complementary receptors on the virus and cell surface draw the membranes close. Subsequently, the membranes begin to merge, leading to a hemifusion state. Finally, a stable entry pore forms, allowing the viral envelope contents to enter the host cell.
Description
This recombinant protein, derived from E. coli, encompasses the C-terminal immunodominant regions of ORF65 (amino acids 140-170) and the N-terminal regions of ORF8 (amino acids 32-62). It is fused with a GST tag.
Purity
The purity of the HSV-8 Mosaic protein exceeds 95%, as determined by 10% SDS-PAGE analysis with Coomassie blue staining.
Physical Appearance
Sterile filtered, clear solution.
Formulation
The protein is formulated in a buffer containing 100mM NaCl, 0.1% SDS, and 50% glycerol.
Stability
For optimal stability, HSV-8 Mosaic protein should be stored below -18°C. While it can remain stable at 4°C for up to one week, repeated freeze-thaw cycles should be avoided.
Applications
HSV-8 Mosaic antigen is well-suited for use in ELISA and Western blot applications. It serves as an excellent antigen for the detection of HSV, exhibiting minimal specificity issues.
Purification Method
HSV-8 Mosaic was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of HSV-8 infected individuals.

Product Science Overview

Introduction

Herpes Simplex Virus-8 (HSV-8), also known as Kaposi’s Sarcoma-associated Herpesvirus (KSHV), is a member of the Herpesviridae family. It is a double-stranded DNA virus that is primarily associated with Kaposi’s Sarcoma, a type of cancer that often affects individuals with compromised immune systems, such as those with HIV/AIDS. The virus is also linked to other malignancies, including primary effusion lymphoma and multicentric Castleman’s disease.

Structure and Composition

The HSV-8 Mosaic Recombinant is a recombinant protein derived from the Herpes Simplex Virus-8. It contains specific immunodominant regions from the viral proteins ORF65 and ORF8. The recombinant protein is typically produced in Escherichia coli (E. coli) and is fused with a Glutathione S-transferase (GST) tag to facilitate purification and detection .

Preparation Methods

The preparation of HSV-8 Mosaic Recombinant involves several steps:

  1. Gene Cloning: The genes encoding the immunodominant regions of ORF65 and ORF8 are cloned into an expression vector that includes a GST tag.
  2. Expression: The recombinant vector is introduced into E. coli cells, which are then cultured to express the recombinant protein.
  3. Purification: The expressed protein is purified using chromatographic techniques, leveraging the GST tag for affinity purification. The resulting protein is typically greater than 95% pure as determined by polyacrylamide gel electrophoresis (PAGE) with Coomassie staining .
Applications

The HSV-8 Mosaic Recombinant protein is widely used in research and diagnostic applications:

  • ELISA and Western Blots: It serves as an antigen for detecting antibodies against HSV-8 in serum samples, making it valuable for diagnostic assays.
  • Immunological Studies: The protein is used to study the immune response to HSV-8, helping to understand the virus’s pathogenesis and immune evasion strategies .
Stability and Storage

The HSV-8 Mosaic Recombinant protein is stable at 4°C for up to one week but should be stored below -18°C for long-term preservation. It is important to avoid repeated freeze-thaw cycles to maintain its integrity .

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