HSV-1 gG
Description
Product Specs
HSV-1 gG was purified by Sepharose-Derived Purification.
Product Science Overview
Herpes Simplex Virus-1 (HSV-1) is a double-stranded DNA virus known for causing oral-facial infections, including cold sores, and can also lead to more severe conditions such as encephalitis. The virus is highly prevalent worldwide and poses significant health challenges. One of the key proteins associated with HSV-1 is glycoprotein G (gG-1), which plays a crucial role in the virus’s ability to infect host cells and evade the immune system.
The recombinant gG-1 protein of HSV-1 is produced using genetic engineering techniques. This involves inserting the gene encoding gG-1 into a suitable expression system, such as Escherichia coli (E. coli), to produce the protein in large quantities. The recombinant gG-1 protein is then purified and used for various applications, including diagnostic assays and vaccine development .
The production of recombinant gG-1 protein typically involves the following steps:
- Gene Cloning: The gene encoding gG-1 is cloned into an expression vector, such as pTrc His2A, which allows for high-level expression in E. coli.
- Transformation: The recombinant plasmid is introduced into E. coli cells through a process called transformation.
- Protein Expression: The transformed E. coli cells are cultured under conditions that induce the expression of the gG-1 protein.
- Protein Purification: The expressed gG-1 protein is purified using techniques such as ion-exchange chromatography, often employing DEAE-sepharose .
The recombinant gG-1 protein is valuable for several reasons:
- Diagnostic Assays: It is used in enzyme-linked immunosorbent assays (ELISA) to detect HSV-1-specific antibodies in patient samples. This helps in distinguishing HSV-1 infections from HSV-2, which is crucial for accurate diagnosis and treatment .
- Vaccine Development: The recombinant protein is also explored as a potential vaccine candidate to elicit an immune response against HSV-1.
- Research: It aids in studying the virus’s biology and interactions with the host immune system, providing insights into potential therapeutic targets .
