HDAC2 Antibody
Histone deacetylase 2, YAF1, HD2, YY1-associated factor 1, transcriptional regulator homolog RPD3, RPD3, EC 3.5.1.98.
Description
Product Specs
Histone deacetylase 2 (HDAC2) is a member of the histone deacetylase family. HDAC2 is involved in the deacetylation of lysine residues on histone proteins. This process is important for regulating gene expression. HDAC2 forms complexes with other proteins, such as YY1, to regulate transcription. HDAC2 plays a crucial role in various cellular processes, including transcriptional regulation, cell cycle progression, and development.
The antibody is supplied at a concentration of 1mg/ml and is dissolved in a buffer solution containing phosphate-buffered saline (PBS) at a pH of 7.4, 10% glycerol, and 0.02% sodium azide.
Repeated freezing and thawing cycles should be avoided.
The HDAC2 antibody has been validated for use in various applications, including ELISA, Western blotting, immunocytochemistry/immunofluorescence (ICC/IF), and flow cytometry. These tests confirm the antibody's specificity and reactivity. However, it is important to note that optimal working dilutions should be determined empirically for each specific application to achieve optimal results.
Histone deacetylase 2, YAF1, HD2, YY1-associated factor 1, transcriptional regulator homolog RPD3, RPD3, EC 3.5.1.98.
HDAC2 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT3B1AT.
Anti-human HDAC2 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HDAC2 amino acids 1-488 purified from E. coli.
Mouse IgG2b heavy chain and κ light chain.
Product Science Overview
Mouse Anti-Human HDAC2 Antibody is a monoclonal antibody derived from mouse hybridoma cells. This antibody is specifically designed to detect human HDAC2 protein and is widely used in various research applications .
Production and Specificity
The antibody is produced by immunizing mice with a synthetic peptide corresponding to the C-terminal region of human HDAC2. The resulting hybridoma cells are then cultured to produce the monoclonal antibody . This antibody recognizes HDAC2 in multiple species, including humans, mice, rats, and chickens .
Applications
Mouse Anti-Human HDAC2 Antibody is used in several laboratory techniques, such as:
- Western Blotting (WB): To detect HDAC2 protein levels in cell extracts .
- Immunoprecipitation (IP): For isolating HDAC2 protein from cell lysates .
- Immunofluorescence (IF): To visualize HDAC2 localization within cells .
- Immunohistochemistry (IHC): For detecting HDAC2 in tissue sections .
Research Significance
This antibody is crucial for studying the role of HDAC2 in gene regulation and its involvement in various diseases. It helps researchers understand the molecular mechanisms underlying HDAC2’s function and its potential as a therapeutic target .
