HCV NS5A

Hepatitis C Virus NS5A Recombinant
Cat. No.
BT17665
Source
Escherichia Coli.
Synonyms
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Recombinant HCV NS5A produced in E. coli is a single polypeptide chain containing 118 amino acids (aa 2210-2306) and having a molecular mass of 13.4kDa.
Recombinant HCV NS5A is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
NS5A is a component of the replication complex that participates in RNA-binding. The hyperphosphorylated form of the NS5A protein inhibits viral replication. NS5A interacts with human VAPB, targeting the viral replication complex to vesicles. Additionally, NS5A downregulates viral IRES translation initiation and mediates interferon resistance by interacting with and inhibiting human EIF2AK2/PKR. NS5a also inhibits apoptosis by interacting with BIN1 and FKBP8.
Description
Recombinant HCV NS5A, produced in E. coli, is a single polypeptide chain consisting of 118 amino acids (aa 2210-2306) with a molecular weight of 13.4 kDa. It is fused to a 21 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The HCV NS5A protein solution (0.25 mg/ml) is supplied in 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), store at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 90% as determined by SDS-PAGE analysis.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSLKATCTAN HDSPDAELIE ANLLWRQEMG GNITRVESEN KVVILDSFDP LVAEEDEREV SVPAEILRKS RRFARALPVW ARPDYNPPLV ETWKKPDY.

Product Science Overview

Introduction

Hepatitis C Virus (HCV) is a significant global health concern, affecting over 150 million people worldwide. It is a major cause of chronic liver diseases, including cirrhosis and hepatocellular carcinoma (HCC) . The HCV genome encodes both structural and nonstructural proteins, which play crucial roles in the virus’s life cycle. Among these, the nonstructural protein 5A (NS5A) is particularly important due to its multifunctional nature and its involvement in viral replication and assembly .

Structure and Function of NS5A

NS5A is a phosphoprotein that consists of three distinct domains, each contributing to different aspects of the viral life cycle . The N-terminal amphipathic alpha-helix (amino acids 5–25) anchors NS5A to the endoplasmic reticulum membrane, facilitating the formation of double-membrane vesicles (DMVs) essential for viral replication .

  • Domain I: Contains zinc-binding and RNA-binding motifs crucial for HCV replication .
  • Domain II: Involved in RNA replication and interacts with various host cell factors .
  • Domain III: Essential for virus assembly and release .
Role in Viral Replication and Assembly

NS5A interacts with other viral proteins, such as NS4B and NS5B, and host cell proteins, including cyclophilin A and various kinases, to regulate viral replication and assembly . It plays a critical role in the formation of DMVs, which provide a conducive environment for efficient viral replication .

Recombinant NS5A

Recombinant NS5A proteins are engineered versions of the natural NS5A protein, often used in research to study the protein’s function and to develop antiviral drugs. These recombinant proteins are produced using various expression systems, such as bacterial, yeast, or mammalian cells, to ensure proper folding and functionality.

Applications in Research and Drug Development

Recombinant NS5A proteins are invaluable tools in the study of HCV biology and the development of antiviral therapies. They are used in:

  • Structural Studies: To understand the protein’s three-dimensional structure and its interactions with other molecules .
  • Drug Screening: To identify and test potential inhibitors of NS5A, which can block viral replication .
  • Vaccine Development: To explore the potential of NS5A as a target for vaccine candidates .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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