HCV NS4, HRP

Hepatitis C Virus NS4 Horseradish Peroxidase Recombinant
Cat. No.
BT15825
Source
Escherichia Coli.
Synonyms
Appearance
Purity
HCV NS4 Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.coli derived recombinant Horseradish Peroxidase Labeled protein contains the HCV NS4 immunodominant regions.

Product Specs

Introduction
Hepatitis C virus (HCV) is a small (50nm), enveloped virus with a single-stranded, positive-sense RNA genome. It belongs to the Flaviviridae family. HCV replicates at a high rate, producing approximately one trillion particles daily in an infected individual. The virus's RNA polymerase lacks proofreading ability, resulting in a high mutation rate, which helps HCV evade the host's immune response. HCV is classified into six genotypes (1-6), each with multiple subtypes. The prevalence and distribution of HCV genotypes vary globally. Genotype determination is crucial clinically as it influences the potential response to interferon-based therapy and the required treatment duration. Genotypes 1 and 4 show a lower response rate to interferon-based treatment compared to genotypes 2, 3, 5, and 6.
Description
This recombinant protein, derived from E. coli, consists of the immunodominant regions of the HCV NS4 protein and is labeled with Horseradish Peroxidase (HRP).
Purity
Analysis by 10% SDS-PAGE and Coomassie staining indicates a purity of greater than 95% for the HCV NS4 protein.
Formulation
The HCV NS4 protein is supplied in a buffer containing 25mM Tris-HCl (pH 8), 1mM EDTA, 1.5M urea, and 50% glycerol.
Stability
For optimal storage, HCV NS4 a+b HRP should be stored below -18°C. While it remains stable at 4°C for up to one week, repeated freeze-thaw cycles should be avoided.
Applications
The HCV NS4 Antigen is well-suited for use in ELISA and Western blot applications. It serves as an excellent antigen for detecting HCV with minimal specificity issues.
Source
Escherichia Coli.
Purification Method
HCV NS4 protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of HCV-infected individuals.

Product Science Overview

Introduction to Hepatitis C Virus (HCV)

Hepatitis C Virus (HCV) is a significant global health concern, affecting millions of individuals worldwide. It is a single-stranded RNA virus belonging to the Flaviviridae family. HCV infection can lead to chronic liver diseases, including cirrhosis and hepatocellular carcinoma. The virus encodes a single polyprotein that is processed into structural and non-structural proteins, including NS4.

NS4 Protein of HCV

The NS4 protein of HCV is a non-structural protein that plays a crucial role in the virus’s replication and assembly. It is divided into two regions: NS4A and NS4B. NS4A acts as a cofactor for the NS3 protease, enhancing its enzymatic activity, while NS4B is involved in the formation of the membranous web, a structure essential for viral replication.

Recombinant Proteins in HCV Research

Recombinant proteins are artificially produced proteins that are used extensively in research and diagnostic applications. In the context of HCV, recombinant proteins derived from various regions of the virus, including NS4, are used to develop assays for detecting HCV antibodies. These assays are crucial for diagnosing HCV infections and monitoring the immune response in infected individuals.

Horseradish Peroxidase (HRP) Conjugation

Horseradish Peroxidase (HRP) is an enzyme commonly used in biochemistry applications, particularly in immunoassays. HRP conjugation involves attaching the HRP enzyme to another molecule, such as an antibody or a recombinant protein. This conjugation allows for the detection of specific antigens or antibodies through colorimetric reactions, making it a valuable tool in diagnostic assays.

HCV NS4 Horseradish Peroxidase Recombinant

The HCV NS4 Horseradish Peroxidase Recombinant is a fusion protein that combines the NS4 region of HCV with the HRP enzyme. This recombinant protein is used in various immunoassays to detect antibodies against HCV in patient samples. The HRP component enables the visualization of the antigen-antibody reaction through a color change, facilitating the identification of HCV infections.

Applications in Diagnostic Assays

The HCV NS4 Horseradish Peroxidase Recombinant is utilized in enzyme-linked immunosorbent assays (ELISAs) and other immunoassays to detect HCV antibodies. These assays are essential for:

  • Screening blood donations to prevent the transmission of HCV through transfusions.
  • Diagnosing HCV infections in individuals, particularly in early stages when symptoms may be mild or absent.
  • Monitoring the immune response in patients undergoing antiviral therapy to assess treatment efficacy.

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THE BIOTEK
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