HCV NS4 a+b Rhodamine

Hepatitis C Virus NS4 a+b Rhodamine Labeled Recombinant
Cat. No.
BT15192
Source
Synonyms
Appearance
Purity
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
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Description

The E.coli derived 19 kDa recombinant protein rhodamine labeled contains the HCV NS4 immunodominant regions, amino acids 1658-1863. The protein is fused with b-galactosidase (114 kDa) at N-terminus.

Product Specs

Introduction
HCV, a member of the Flaviviridae family, is a small (50nm), enveloped, single-stranded RNA virus with a positive sense. It exhibits a high replication rate, producing approximately one trillion particles daily in an infected individual. The HCV RNA polymerase's lack of proofreading results in an exceptionally high mutation rate, potentially contributing to its ability to evade the host's immune response. HCV is classified into six genotypes (1-6), each with several subtypes. The prevalence and distribution of these genotypes vary globally. Genotype plays a crucial role in determining the potential response to interferon-based therapy and the required treatment duration. Notably, genotypes 1 and 4 show less responsiveness to interferon-based treatment compared to genotypes 2, 3, 5, and 6.
Description
This recombinant protein, derived from E. coli, is 19 kDa in size and contains the HCV NS4 immunodominant regions (amino acids 1658-1863). It is labeled with rhodamine for detection purposes. The protein is expressed as a fusion with b-galactosidase (114 kDa) at its N-terminus.
Purity
The purity of this protein is greater than 95% as determined by 10% SDS-PAGE analysis with Coomassie blue staining.
Formulation
The protein is supplied in a buffer consisting of 20mM Tris-HCl (pH 8), 8M urea, and 10mM beta-mercaptoethanol (B-ME).
Stability
HCV NS4 a+b Rhodamine, although stable at 4°C for one week, is best stored below -18°C to ensure long-term stability. Avoid repeated freeze-thaw cycles.
Applications
This protein serves as an antigen in various applications, including enzyme-linked immunosorbent assays (ELISA) and Western blots. It is particularly effective for HCV detection with minimal specificity issues.
Purification Method

Purified by proprietary chromatographic technique.

Specificity
Immunoreactive with sera of HCV-infected individuals.

Product Science Overview

Introduction

Hepatitis C Virus (HCV) is a significant global health concern, affecting millions of people worldwide. The virus is known for causing chronic liver diseases, including cirrhosis and hepatocellular carcinoma. The HCV genome encodes a single polyprotein that is processed into structural and non-structural proteins. Among these, the non-structural protein 4 (NS4) plays a crucial role in the virus’s life cycle and pathogenesis.

NS4 Protein

The NS4 protein of HCV is divided into two regions: NS4A and NS4B. NS4A acts as a cofactor for the NS3 serine protease, which is essential for the cleavage of the non-structural region of the polyprotein . NS4B, on the other hand, is involved in the formation of the membranous web, a structure critical for HCV RNA replication . Together, these proteins contribute to the virus’s ability to replicate and evade the host immune response.

Recombinant NS4 Protein

Recombinant NS4 proteins are produced using various expression systems, with Escherichia coli being one of the most common . These recombinant proteins are often used in research to study the virus’s biology and to develop diagnostic assays and potential therapeutic interventions. The recombinant NS4 protein is typically expressed with a high degree of purity, often exceeding 95% .

Rhodamine Labeling

Rhodamine is a fluorescent dye commonly used in biological research. When conjugated to proteins, it allows for the visualization and tracking of these proteins in various experimental setups. Rhodamine-labeled recombinant NS4 proteins are particularly useful in studying the interactions and localization of the NS4 protein within host cells .

Applications
  1. Diagnostic Assays: Rhodamine-labeled NS4 proteins can be used in immunoassays to detect antibodies against HCV in patient samples. This is crucial for diagnosing HCV infections and monitoring the immune response during treatment .
  2. Research: These labeled proteins are valuable tools for studying the intracellular localization and interactions of NS4 proteins. They help in understanding the mechanisms of HCV replication and pathogenesis .
  3. Therapeutic Development: By studying the interactions of NS4 proteins with host cell factors, researchers can identify potential targets for antiviral drugs. Rhodamine labeling aids in visualizing these interactions, facilitating the development of new therapeutic strategies .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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