HCV NS3 Genotype-6
Description
Product Specs
Product Science Overview
Hepatitis C Virus (HCV) is a significant global health concern, affecting millions of people worldwide. It is a leading cause of chronic liver diseases, including liver cirrhosis and hepatocellular carcinoma. The virus is classified into several genotypes, with genotype 6 being prevalent in Southeast Asia. The non-structural protein 3 (NS3) of HCV plays a crucial role in the viral life cycle, making it a target for antiviral therapies.
The NS3 protein is a multifunctional enzyme with protease, helicase, and nucleoside triphosphatase (NTPase) activities. It is essential for the processing of the HCV polyprotein and the replication of the viral RNA. The NS3 protein is composed of two domains: the N-terminal protease domain and the C-terminal helicase domain. The protease domain is responsible for cleaving the viral polyprotein into functional units, while the helicase domain unwinds the RNA duplexes during replication.
HCV genotype 6 is predominantly found in Southeast Asia and is known for its genetic diversity. The NS3 protein of genotype 6 has unique sequence variations that distinguish it from other genotypes. These variations can influence the protein’s structure and function, affecting the virus’s replication efficiency and response to antiviral treatments.
Recombinant NS3 protein refers to the NS3 protein that has been artificially produced using recombinant DNA technology. This involves cloning the NS3 gene into an expression vector, introducing the vector into a host cell (such as E. coli), and inducing the expression of the NS3 protein. The recombinant protein can then be purified and used for various applications, including structural studies, drug screening, and vaccine development.
- Drug Development: The NS3 protein is a target for direct-acting antiviral agents (DAAs). Inhibitors of the NS3 protease have been developed and are used in combination therapies to treat HCV infections. Studying the recombinant NS3 protein helps in understanding the enzyme’s structure and function, facilitating the design of more effective inhibitors.
- Vaccine Development: The recombinant NS3 protein can be used as an antigen in vaccine formulations. It can elicit an immune response in the host, providing protection against HCV infection. Research on the antigenic properties of the NS3 protein from different genotypes, including genotype 6, is crucial for developing a broad-spectrum HCV vaccine.
- Diagnostic Tools: Recombinant NS3 protein can be used in diagnostic assays to detect HCV infection. Enzyme immunoassays (EIAs) and other serological tests can utilize the recombinant protein to identify antibodies against HCV in patient samples, aiding in the early diagnosis and management of the disease.
