HCV NS3 Genotype-5

Hepatitis C Virus NS3 Genotype-5, (1192-1459 a.a.) Recombinant
Cat. No.
BT14558
Source
Synonyms
Appearance
Purity
HCV NS3 Genotype-5 protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.coli derived recombinant protein fused to a His tag contains the HCV NS3 immunodominant regions, amino acids 1192-1459.

Product Specs

Introduction
Hepatitis C virus (HCV) is a small (50nm), enveloped, single-stranded RNA virus with a positive sense genome. It belongs to the family Flaviviridae. HCV replicates at a high rate, producing approximately one trillion particles daily in an infected individual. The virus's RNA polymerase lacks proofreading ability, resulting in a high mutation rate, which contributes to its ability to evade the host's immune system. HCV is categorized into six genotypes (1-6), each with multiple subtypes. The prevalence and distribution of these genotypes vary globally. Genotype determination is clinically significant as it influences the potential response to interferon-based therapy and the required treatment duration. Genotypes 1 and 4 exhibit lower responsiveness to interferon-based treatment compared to genotypes 2, 3, 5, and 6.
Description
This recombinant protein is derived from E. coli and is fused to a His tag. It encompasses the immunodominant regions of the HCV NS3 protein, specifically amino acids 1192-1459.
Purity
The purity of the HCV NS3 Genotype-5 protein exceeds 95%, as determined by 10% PAGE (coomassie staining).
Formulation
The protein is supplied in a buffer containing 1.5M urea, 25mM Tris-HCl (pH 8), 0.2% Triton-X, and 50% glycerol.
Stability
For optimal stability, HCV NS3 Genotype-5 should be stored below -18°C. While the protein remains stable at 4°C for up to one week, it is recommended to avoid repeated freeze-thaw cycles.
Applications
The HCV NS3 Genotype-5 antigen is suitable for use in ELISA and Western blot assays. It serves as an excellent antigen for HCV detection with minimal specificity issues.
Purification Method
HCV NS3 Genotype-5 protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of HCV-infected individuals.

Product Science Overview

Introduction

Hepatitis C Virus (HCV) is a significant global health concern, affecting millions of people worldwide. The virus can lead to severe liver diseases, including cirrhosis and hepatocellular carcinoma. Among the various genotypes of HCV, genotype 5 is less common but still poses a considerable health risk. The non-structural protein 3 (NS3) of HCV plays a crucial role in the virus’s life cycle and is a target for antiviral therapies.

Structure and Function of NS3

The NS3 protein is a multifunctional enzyme with protease, helicase, and nucleoside triphosphatase (NTPase) activities. It is essential for the processing of the HCV polyprotein and the replication of the viral RNA. The NS3 protein consists of two domains: the N-terminal protease domain and the C-terminal helicase domain. The protease domain is responsible for cleaving the HCV polyprotein into functional units, while the helicase domain unwinds the RNA duplexes, facilitating replication.

Recombinant NS3 Protein

The recombinant NS3 protein, specifically the segment from amino acids 1192 to 1459, is often used in research and diagnostic applications. This segment includes the immunodominant regions of the protein, making it highly reactive with antibodies from HCV-infected individuals. The recombinant protein is typically expressed in bacterial systems, such as Escherichia coli, and purified using affinity chromatography techniques .

Applications in Research and Diagnostics

The recombinant NS3 protein is valuable in several research and diagnostic contexts:

  • Vaccine Development: The immunogenic properties of the NS3 protein make it a potential candidate for vaccine development. Researchers are exploring ways to elicit a robust immune response against this protein to prevent HCV infection.
  • Antiviral Drug Screening: The enzymatic activities of NS3 make it an attractive target for antiviral drugs. Inhibitors of the NS3 protease and helicase are being developed and tested for their efficacy in blocking HCV replication.
  • Diagnostic Assays: The recombinant NS3 protein is used in enzyme-linked immunosorbent assays (ELISAs) and Western blotting to detect antibodies against HCV in patient sera. These assays are crucial for the early diagnosis and monitoring of HCV infection .

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