HCV NS3 Genotype-4c
Description
Product Specs
Product Science Overview
Hepatitis C Virus (HCV) is a significant global health concern, affecting millions of people worldwide. The virus is known for causing chronic liver diseases, including cirrhosis and hepatocellular carcinoma. Among the various genotypes of HCV, genotype 4 is predominantly found in the Middle East and Africa. The nonstructural protein 3 (NS3) of HCV plays a crucial role in the viral life cycle, making it a target for therapeutic interventions.
The NS3 protein of HCV is a multifunctional enzyme with protease, helicase, and RNA-stimulated NTPase activities. It is involved in the processing of the viral polyprotein and the replication of the viral RNA. The NS3 protein is composed of two domains: the N-terminal protease domain and the C-terminal helicase domain. The protease domain is responsible for cleaving the viral polyprotein, while the helicase domain unwinds the RNA, facilitating replication .
Genotype 4c is a subtype of HCV genotype 4, which is less studied compared to other genotypes like 1 and 3. The NS3 protein of genotype 4c has unique amino acid sequences that may influence its enzymatic activities and interactions with host proteins. Understanding these differences is essential for developing genotype-specific antiviral therapies.
Recombinant proteins are artificially produced proteins that are used for various research and therapeutic purposes. The recombinant NS3 protein of HCV genotype-4c, specifically the region spanning amino acids 1356-1459, is of particular interest. This region includes critical functional sites that are essential for the protease and helicase activities of NS3 .
The recombinant NS3 protein is typically expressed in bacterial systems, such as Escherichia coli, using plasmid vectors. The expression of the protein is induced, and the cells are lysed to extract the protein. The recombinant protein is then purified using techniques like affinity chromatography. The purity and activity of the protein are confirmed through various biochemical assays .
The recombinant NS3 protein is used in several research applications, including the study of its enzymatic activities, interactions with other viral and host proteins, and the development of inhibitors. It is also used in the development of diagnostic assays and vaccines. The study of the NS3 protein helps in understanding the mechanisms of HCV replication and pathogenesis, which is crucial for developing effective antiviral therapies .
