HCV NS3 2c

Hepatitis C Virus NS3 Genotype-2c, (1356-1459 a.a.) Recombinant
Cat. No.
BT13715
Source
Synonyms
Appearance
Purity
HCV NS3 Genotype-2c protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.coli derived recombinant protein contains the HCV NS3 immunodominant regions, amino acids 1356-1459.

Product Specs

Introduction
Hepatitis C virus (HCV) is a small (50nm), enveloped virus with a single-stranded, positive-sense RNA genome. It belongs to the family Flaviviridae. HCV replicates rapidly, producing approximately one trillion particles daily in infected individuals. The virus exhibits a high mutation rate due to the lack of proofreading mechanisms in its RNA polymerase, contributing to its ability to evade the host's immune system. HCV is classified into six genotypes (1-6), each with several subtypes. The prevalence and distribution of HCV genotypes vary globally. Genotype determination is crucial for guiding treatment strategies, as it influences the response to interferon-based therapy and the required treatment duration. Notably, genotypes 1 and 4 demonstrate lower responsiveness to interferon-based treatments compared to genotypes 2, 3, 5, and 6.
Description
This recombinant protein is derived from E. coli and encompasses the immunodominant regions of the HCV NS3 protein, specifically amino acids 1356-1459.
Purity
The purity of HCV NS3 Genotype-2c protein exceeds 95%, as determined by 10% SDS-PAGE analysis with Coomassie blue staining.
Formulation
The protein is supplied in a buffer consisting of 1.5M urea, 25mM Tris-HCl (pH 8.0), 0.2% Triton X-100, and 50% glycerol.
Stability
HCV NS3 Genotype-2c remains stable for up to 1 week when stored at 4°C. For long-term storage, it is recommended to store the protein below -18°C. Avoid repeated freeze-thaw cycles.
Applications
HCV NS3 Genotype-2c antigen is suitable for various applications, including ELISA and Western blotting. It exhibits excellent antigenicity for HCV detection, demonstrating minimal cross-reactivity with other proteins.
Purification Method
HCV NS3 Genotype-2c protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of HCV-infected individuals.

Product Science Overview

Introduction

Hepatitis C Virus (HCV) is a significant global health concern, affecting millions of individuals worldwide. The virus is classified into six major genotypes, each with several subtypes. Among these, Genotype 2c is one of the less common but clinically important variants. The nonstructural protein 3 (NS3) of HCV plays a crucial role in the virus’s life cycle and is a target for antiviral therapies.

Structure and Function of NS3

The NS3 protein is a multifunctional enzyme with protease, helicase, and nucleoside triphosphatase activities. It is essential for the processing of the HCV polyprotein and the replication of the viral RNA. The NS3 protein, along with its cofactor NS4A, cleaves the viral polyprotein at specific sites to produce mature viral proteins necessary for the assembly of new virions .

Genotype-2c Specifics

Genotype 2c is one of the subtypes of HCV Genotype 2. It is less prevalent compared to Genotype 1 but is known for its better response to interferon-based therapies. The NS3 protein of Genotype 2c, particularly the region spanning amino acids 1356 to 1459, has been studied for its unique structural and functional properties .

Recombinant NS3 Protein

Recombinant NS3 proteins are produced using various expression systems, such as bacterial, yeast, or mammalian cells. These recombinant proteins are used in research to study the enzyme’s structure, function, and interactions with antiviral drugs. The recombinant NS3 protein of HCV Genotype-2c (1356-1459 a.a.) is particularly valuable for developing and testing new therapeutic agents .

Applications in Research and Medicine
  1. Drug Development: The NS3 protein is a target for direct-acting antiviral agents (DAAs). Inhibitors of the NS3 protease have been developed and are used in combination therapies to treat HCV infections.
  2. Vaccine Research: Recombinant NS3 proteins are used to develop vaccines that elicit immune responses against HCV. These vaccines aim to prevent infection or reduce the severity of the disease.
  3. Diagnostic Tools: NS3 proteins are also used in diagnostic assays to detect HCV infections and monitor the effectiveness of antiviral treatments .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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