HBsAgA Antibody

Hepatitis B surface Antigen A, Polyclonal Goat Antibody
Cat. No.
BT4078
Source
Synonyms
Appearance
Sterile Filtered solution.
Purity
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

Affinity-purified Goat antibodies against the Native Ad/AyHBsAg antigens.1mg/ml in PBS, was passed through a 0.2um Filter into a 50ml sterile conic Tube.

Product Specs

Introduction
Hepatitis B virus (HBV) is unusual in that it is a non-retrovirus that uses reverse transcription during replication. This process is also used by HIV, a completely unrelated virus that is classified as a retrovirus. HBV infection begins when the virus binds to surface receptors on hepatocytes and enters the cells. The viral core particles then travel to the hepatocyte nucleus. Here, the partially double-stranded, relaxed circular DNA (RC-DNA) genomes are repaired to form covalently closed circular DNA (cccDNA), which acts as the template for viral genomic and sub-genomic RNA production by cellular RNA polymerase II. Of these RNA molecules, the pregenomic RNA (pgRNA) is selectively packaged into progeny capsids. Inside the capsids, the pgRNA is reverse-transcribed into new RC-DNA. The core can then either bud into the endoplasmic reticulum, where it is enveloped and exported from the cell, or it can be recycled back into the genome for conversion to cccDNA.
Description
This product contains 1 mg/mL of affinity-purified goat antibodies against native Ad/Ay HBsAg antigens. The antibodies are supplied in PBS and have been sterile-filtered through a 0.2 μm filter. The product is supplied in a 50 mL sterile conical tube.
Physical Appearance
Sterile-filtered solution
Formulation
1 mL of PBS
Storage Procedures
Store at -20°C.
Stability / Shelf Life
The product is stable for one year when stored frozen at -20°C. Once thawed, the product is stable for one month when stored at 4°C.
Specificity
Immunoreactive with all HBsAg A antigens. Direct ELISA shows signal above cut-off at antibody levels of 100ng/test and above.
Type
Polyclonal Goat Antibody.
Host
Rabbit.

Product Science Overview

Hepatitis B Surface Antigen (HBsAg)

Hepatitis B surface antigen (HBsAg) is a protein found on the surface of the hepatitis B virus (HBV). It is one of the earliest markers to appear in the blood following HBV infection and is used to diagnose acute and chronic hepatitis B infections . HBsAg can be detected as early as one week after exposure to the virus and typically disappears within 15 weeks in those who recover .

Polyclonal Antibodies

Polyclonal antibodies are a mixture of antibodies produced by different B cell clones in the body. They recognize and bind to multiple epitopes on a single antigen, providing a robust and versatile immune response. Polyclonal antibodies are commonly used in research, diagnostics, and therapeutic applications due to their ability to recognize multiple sites on an antigen.

Production of Polyclonal Goat Antibodies

Polyclonal antibodies can be produced in various animals, including goats. The process involves immunizing the goat with the antigen of interest, in this case, HBsAg. The goat’s immune system responds by producing antibodies against the antigen. After a series of immunizations, blood is collected from the goat, and the antibodies are purified from the serum.

Applications of Polyclonal Goat Antibodies Against HBsAg

Polyclonal goat antibodies against HBsAg are used in various applications, including:

  1. Diagnostic Testing: These antibodies are used in enzyme-linked immunosorbent assays (ELISAs) and other immunoassays to detect HBsAg in blood samples. This helps in diagnosing hepatitis B infections .
  2. Research: Researchers use these antibodies to study the structure and function of HBsAg, as well as the immune response to HBV infection .
  3. Therapeutics: Polyclonal antibodies can be used in passive immunization to provide immediate protection against HBV infection. They are also being explored as potential therapeutic agents to neutralize the virus and prevent its spread.

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