HBsAg adw2

Hepatitis B Surface Antigen (HBsAg) is a protein component of the Hepatitis B virus (HBV) envelope. It plays a crucial role in the virus’s ability to infect liver cells and is a key target for diagnostic tests and vaccines. The “adw2” subtype refers to a specific variant of HBsAg, which is used in various research and clinical applications.

The recombinant form of HBsAg, adw2, is produced using genetic engineering techniques. The gene encoding the HBsAg is inserted into the DNA of a host organism, typically yeast cells such as Saccharomyces cerevisiae or Pichia pastoris. These genetically modified cells then express the HBsAg protein, which can be harvested and purified for use .

The recombinant HBsAg, adw2, undergoes several purification steps to ensure high purity and quality. Common methods include ionic exchange chromatography, size exclusion chromatography, and sterile filtration. The final product is often formulated in a phosphate buffer solution with sodium chloride to maintain stability and activity .

1. Vaccines: Recombinant HBsAg is a critical component of hepatitis B vaccines. It stimulates the immune system to produce antibodies against HBV, providing protection against infection. For example, the ENGERIX-B vaccine contains purified HBsAg produced in Saccharomyces cerevisiae .
2. Diagnostic Tests: HBsAg is used in diagnostic assays to detect HBV infection. These tests can identify the presence of HBsAg in blood samples, indicating an active or past infection.
3. Research: Recombinant HBsAg is used in various research applications, including studying the structure and function of the HBV envelope, developing new diagnostic methods, and testing antiviral drugs .

Recombinant HBsAg, adw2, should be stored at 4°C and should not be frozen to maintain its stability and activity. The protein is typically provided in a sterile filtered solution to prevent contamination .