HB-EGF Mouse
DTR, HEGFL, diphtheria toxin receptor, DTSF.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
The HB-EGF is purified by proprietary chromatographic techniques
Product Specs
Heparin-binding EGF-like growth factor (HB-EGF) is a member of the epidermal growth factor (EGF) family that binds to and activates the EGF receptor (EGFR), leading to the stimulation of cell growth and proliferation. HB-EGF is produced by a variety of cell types, including smooth muscle cells, fibroblasts, epithelial cells, and keratinocytes. It plays a role in a variety of biological processes, including wound healing, angiogenesis, and tumorigenesis. One unique feature of HB-EGF is its ability to bind to sulfate proteoglycans, which may contribute to its enhanced mitogenic activity compared to EGF.
DTR, HEGFL, diphtheria toxin receptor, DTSF.
Product Science Overview
HB-EGF is initially synthesized as a transmembrane precursor, which can be cleaved to release a soluble form. Both the transmembrane and soluble forms of HB-EGF are biologically active. The transmembrane form of HB-EGF is unique in that it serves as the receptor for diphtheria toxin and functions in juxtacrine signaling, which involves direct cell-to-cell communication .
HB-EGF binds to two primary locations on cell surfaces: heparan sulfate proteoglycans and EGF receptors. This binding facilitates cell-to-cell interactions and plays a crucial role in various physiological and pathological processes .
HB-EGF has been shown to play significant roles in:
- Wound Healing: It promotes the proliferation and migration of keratinocytes and fibroblasts, which are essential for wound repair.
- Cardiac Hypertrophy and Heart Development: HB-EGF is involved in the development and function of the heart, including the formation of cardiac valves and the regulation of cardiac hypertrophy.
- Smooth Muscle Cell Proliferation: It stimulates the proliferation of smooth muscle cells, which is important for vascular development and repair .
HB-EGF is implicated in several pathological conditions, including:
- Tumor Progression and Metastasis: It contributes to the growth and spread of tumors by promoting cell proliferation and migration.
- Organ Hyperplasia: HB-EGF is involved in the abnormal enlargement of organs due to an increase in cell number.
- Atherosclerotic Disease: It plays a role in the development of atherosclerosis by stimulating the proliferation of smooth muscle cells within blood vessels .
Recombinant HB-EGF (Mouse) is produced using an expression system in Escherichia coli (E. coli). The recombinant protein is a single non-glycosylated polypeptide chain containing 86 amino acids. It is fully biologically active and has a specific activity of greater than 1.0 × 10^6 IU/mg as determined by a cell proliferation assay using murine Balb/c 3T3 cells .
The recombinant protein is typically lyophilized and can be reconstituted in sterile distilled water or an aqueous buffer containing 0.1% bovine serum albumin (BSA) to a concentration of 0.1-1.0 mg/mL. It is stable for up to six months when stored at -70°C or -20°C and for up to one week at 4°C after reconstitution .
