HB-EGF Human, His

Proheparin-Binding EGF-like Growth Factor Human Recombinant, His Tag
Cat. No.
BT4439
Source
Escherichia Coli.
Synonyms
Proheparin-binding EGF-like growth factor, HBEGF, DTR, DTS, HEGFL, HB-EGF, Heparin-binding EGF-like growth factor, Diphtheria toxin receptor, DT-R, DTSF.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Shipped with Ice Packs
In Stock
Quick Inquiry

Description

HB-EGF His Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 109 amino acids (63-148) and having a molecular mass of 12.1kDa.
HB-EGF His is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Heparin-binding EGF-like growth factor (HB-EGF) is a member of the epidermal growth factor (EGF) family that binds to and activates the EGF receptor (EGFR). HB-EGF stimulates the proliferation of a variety of cell types, including smooth muscle cells, fibroblasts, epithelial cells, and keratinocytes. It is expressed in a variety of tissues, including vascular endothelial cells, smooth muscle cells, macrophages, skeletal muscle, keratinocytes, and some tumor cells. Unlike other EGF-like molecules, HB-EGF can bind to heparin and heparan sulfate proteoglycans, which may contribute to its enhanced mitogenic activity on smooth muscle cells compared to EGF.
Description
Recombinant human HB-EGF His is a single, non-glycosylated polypeptide chain containing amino acids 63-148 (109 amino acids total) with a molecular mass of 12.1 kDa. The protein is expressed in E. coli with an N-terminal 23 amino acid His-tag and purified by proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The HB-EGF His solution is provided at a concentration of 0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 2 mM DTT, and 50% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, store frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage. Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% purity as determined by SDS-PAGE analysis.
Synonyms
Proheparin-binding EGF-like growth factor, HBEGF, DTR, DTS, HEGFL, HB-EGF, Heparin-binding EGF-like growth factor, Diphtheria toxin receptor, DT-R, DTSF.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSDLQEADL DLLRVTLSSK PQALATPNKE EHGKRKKKGK GLGKKRDPCL RKYKDFCIHG ECKYVKELRA PSCICHPGYH GERCHGLSL.

Product Science Overview

Introduction

Proheparin-Binding EGF-like Growth Factor (HB-EGF) is a member of the EGF family of mitogens. It is a potent growth factor that plays a crucial role in various cellular processes, including cell proliferation, differentiation, and migration. The recombinant form of HB-EGF, tagged with a His tag, is widely used in research and therapeutic applications.

Structure and Function

HB-EGF is initially synthesized as a transmembrane protein, which is then cleaved to release the soluble form. The soluble form of HB-EGF can bind to and activate the epidermal growth factor receptor (EGFR), as well as ERBB2 and ERBB4 receptors . This binding triggers a cascade of downstream signaling pathways that promote cell proliferation and survival.

Biological Roles

HB-EGF is involved in several physiological and pathological processes:

  • Cardiac Development: It is essential for normal cardiac valve formation and heart function .
  • Wound Healing: HB-EGF promotes the proliferation of smooth muscle cells, epithelial cells, and keratinocytes, which are crucial for wound healing .
  • Macrophage-Mediated Proliferation: It may play a role in macrophage-mediated cellular proliferation .
Recombinant HB-EGF (His Tag)

The recombinant form of HB-EGF, tagged with a His tag, is produced using recombinant DNA technology. The His tag facilitates the purification of the protein using affinity chromatography. This form is used in various research applications to study the biological functions of HB-EGF and its interactions with other molecules.

Applications

Recombinant HB-EGF (His Tag) is used in:

  • Cell Culture: To stimulate the proliferation of various cell types, including smooth muscle cells, fibroblasts, and epithelial cells .
  • Therapeutic Research: Investigating its potential in wound healing and tissue regeneration.
  • Molecular Biology Studies: Understanding the signaling pathways mediated by HB-EGF and its receptors.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

HB-EGF Human
HB-EGF Human Recombinant
Cat. No.
BT4312
Source
Escherichia Coli.
HB-EGF Mouse
HB-EGF Mouse Recombinant
Cat. No.
BT4525
Source
Escherichia Coli.
HB-EGF Rat
Proheparin-Binding EGF-like Growth Factor Rat Recombinant
Cat. No.
BT4595
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.