HAX1 Human
HCLS1 Associated Protein X-1 Human Recombinant
Cat. No.
BT14538
Source
E.coli.
Synonyms
HCLS1-associated protein X-1, HS1-associating protein X-1, HS1-binding protein 1, HAX-1, HSP1BP-1, HAX1, HS1BP1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
HAX1 Human Recombinant produced in E. coli is a single polypeptide chain containing 299 amino acids (1-279) and having a molecular mass of 33.7kDa. HAX1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
HAX1, also known as HCLS1-associated protein X-1, is a protein involved in various cellular processes. It interacts with proteins such as Lyn substrate 1 (a substrate of Src family tyrosine kinases), polycystic kidney disease 2 gene product (associated with autosomal-dominant polycystic kidney disease), and cortactin (an F-actin-binding protein). Initially thought to be primarily located in mitochondria, HAX1 is now understood to be present in the cell body. Mutations in the HAX1 gene are linked to a severe congenital neutropenia known as Kostmann disease.
Description
This product consists of the recombinant human HAX1 protein, expressed in E. coli. It is a single polypeptide chain with a molecular weight of 33.7kDa, comprising 299 amino acids (residues 1-279). A 20 amino acid His-tag is fused to the N-terminus of the protein. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
A clear and colorless solution, sterilized by filtration.
Formulation
The HAX1 solution is provided at a concentration of 0.5mg/ml. It is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 10% glycerol, and 0.1M NaCl.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the HAX1 protein is determined to be greater than 80% using SDS-PAGE analysis.
Synonyms
HCLS1-associated protein X-1, HS1-associating protein X-1, HS1-binding protein 1, HAX-1, HSP1BP-1, HAX1, HS1BP1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSLFDLFRGF FGFPGPRSHR DPFFGGMTRD EDDDEEEEEE GGSWGRGNPR FHSPQHPPEE FGFGFSFSPG GGIRFHDNFG FDDLVRDFNS IFSDMGAWTL PSHPPELPGP ESETPGERLR EGQTLRDSML KYPDSHQPRI FGGVLESDAR SESPQPAPDW GSQRPFHRFD DVWPMDPHPR TREDNDLDSQ VSQEGLGPVL QPQPKSYFKS ISVTKITKPD GIVEERRTVV DSEGRTETTV TRHEADSSPR GDPESPRPPA LDDAFSILDL FLGRWFRSR.
Product Science Overview
Introduction
HCLS1-associated protein X-1 (HAX1) is an intracellular anti-apoptosis protein discovered by Suzuki et al. in 1997 through a yeast two-hybrid screening study . The protein primarily interacts with hematopoietic cell-specific Lyn substrate 1 (HCLS1), also known as HS1. HAX1 is widely expressed in various tissues and cells, showing a punctate distribution mainly in the mitochondria, with some presence around the endoplasmic reticulum and nuclear membrane .
Biological Functions
HAX1 is a multifunctional protein that plays crucial roles in several cellular processes:
- Regulation of Apoptosis: HAX1 interacts with various intracellular apoptosis-related proteins, such as caspase 3 and 9, and high-temperature requirement protein A2 (HtrA2), to regulate cellular apoptosis .
- Mitochondrial Membrane Potential Stability: It helps maintain the stability of the mitochondrial membrane potential and calcium homeostasis .
- Disease Progression: HAX1 is involved in the occurrence and development of diseases, including tumor occurrence and migration .
- Gene Expression Regulation: It participates in post-transcriptional regulation of gene expression and RNA transcription and translation .
- Host Immune Response: HAX1 can bind to viral proteins and participate in the processes of viral infection and host response .
Expression and Subcellular Localization
Interaction with Other Proteins
HAX1 interacts with a variety of proteins to exert its biological functions:
- Viral Proteins: It can bind to proteins of viruses such as Kaposi’s sarcoma-associated herpes virus (KSHV), human immunodeficiency virus (HIV), and Epstein–Barr virus (EBV), regulating apoptosis after viral infection and affecting viral replication .
- HCLS1 and LEF-1: HAX1 interacts with HCLS1 and lymphoid-enhancer binding factor 1 (LEF-1), which are essential for granulocyte colony-stimulating factor (G-CSF)-triggered granulopoiesis .
Clinical Significance
Mutations in the gene encoding HAX1 are associated with severe congenital neutropenia, a condition characterized by a lack of neutrophils, leading to increased susceptibility to infections . HAX1’s role in maintaining mitochondrial membrane potential and regulating apoptosis makes it a potential target for therapeutic interventions in various diseases, including cancer and viral infections .
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