HAX1 Antibody

HCLS1-associated protein X-1, Mouse Anti Human
Cat. No.
BT19515
Source
Synonyms
HCLS1-associated protein X-1, HS1-associating protein X-1, HS1-binding protein 1, HAX-1, HSP1BP-1, HAX1, HS1BP1.
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Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals
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Description

Product Specs

Introduction
HAX1 is a protein that interacts with several other proteins, including hematopoietic cell-specific Lyn substrate 1 (HCLS1), polycystic kidney disease 2 gene product (PKD2), and cortactin. HAX1 was initially thought to be primarily located in mitochondria but is now understood to be present throughout the cell. Mutations in the HAX1 gene are linked to severe congenital neutropenia, an autosomal recessive disorder also known as Kostmann disease.
Formulation

The antibody is supplied in a solution containing 1mg/ml of antibody in PBS at a pH of 7.4 with 0.1% sodium azide added as a preservative.

Storage Procedures
For short-term storage (up to 1 month), keep at 4°C. For longer storage, store at -20°C. Avoid repeated freezing and thawing.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This HAX1 antibody has undergone testing via ELISA and Western blot analysis to ensure its specificity and reactivity. However, optimal working dilutions should be determined empirically for different applications. For Western blot analysis, a dilution range of 1:1000 to 1:2000 is recommended, with a starting dilution of 1:1000.
Synonyms
HCLS1-associated protein X-1, HS1-associating protein X-1, HS1-binding protein 1, HAX-1, HSP1BP-1, HAX1, HS1BP1.
Purification Method
HAX1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT3C5AT.
Immunogen
Anti-human HAX1 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HAX1 amino acids 1-279 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.

Product Science Overview

Introduction

HCLS1-associated protein X-1 (HAX1) is a multifunctional intracellular protein that plays a crucial role in various cellular processes. It was initially discovered by Suzuki et al. using the yeast two-hybrid system, which identified its interaction with hematopoietic cell-specific protein 1 (HCLS1) . HAX1 is widely expressed in different tissues and cells, predominantly localized in the mitochondria, with some distribution around the endoplasmic reticulum and nuclear membrane .

Biological Functions

HAX1 is involved in several critical cellular functions, including:

  • Apoptosis Regulation: HAX1 acts as an anti-apoptotic factor, interacting with apoptosis-related proteins such as caspase 3 and 9, and high-temperature requirement protein A2 (HtrA2) to regulate cell death .
  • Calcium Homeostasis: It plays a role in maintaining calcium homeostasis within cells, which is essential for various cellular activities .
  • Cell Migration: HAX1 is involved in the regulation of cell migration, which is crucial for processes like wound healing and cancer metastasis .
  • Mitochondrial Membrane Potential: It helps stabilize the mitochondrial membrane potential, which is vital for energy production and cell survival .
Disease Associations

HAX1 has been implicated in several diseases:

  • Neutropenia: Mutations in HAX1 can lead to severe congenital neutropenia (Kostmann disease), characterized by a deficiency of neutrophils, a type of white blood cell . This condition can result in severe infections and other complications.
  • Cancer: Overexpression of HAX1 has been reported in various cancers, including breast cancer, where it is thought to contribute to tumor progression and metastasis .
  • Psoriasis: Elevated levels of HAX1 have also been observed in psoriasis, a chronic inflammatory skin condition .
Interactions and Mechanisms

HAX1 interacts with a wide range of proteins, forming a complex interactome that is cell-specific . This protein is considered an intrinsically disordered protein (IDP), meaning it does not have a fixed three-dimensional structure but can interact with multiple partners . Some of the key interactions include:

  • Viral Proteins: HAX1 can bind to proteins from viruses such as Kaposi’s sarcoma-associated herpes virus (KSHV), human immunodeficiency virus (HIV), and Epstein–Barr virus (EBV), influencing viral replication and host immune response .
  • Cellular Proteins: It interacts with various cellular proteins involved in apoptosis, calcium signaling, and cytoskeleton organization .

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HAX1 Human
HCLS1 Associated Protein X-1 Human Recombinant
Cat. No.
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Source
E.coli.
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