Anhui H7N9
Sf9, Baculovirus cells.
Hemagglutinin, Influenza A virus (A/Anhui/1-BALF_RG45/2013(H7N9) hemagglutinin, HA, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
Greater than 95.0% as determined by SDS-PAGE.
Description
Anhui H7N9 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 330 amino acids (19-339 aa) and having a molecular mass of 36 kDa.
Anhui H7N9 is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Hemagglutinin, Influenza A virus (A/Anhui/1-BALF_RG45/2013(H7N9) hemagglutinin, HA, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
Sf9, Baculovirus cells.
ADLDKICLGH HAVSNGTKVN TLTERGVEVV NATETVERTN IPRICSKGKR TVDLGQCGLL GTITGPPQCD QFLEFSADLI IERREGSDVC YPGKFVNEEA LRQILRESGG IDKEAMGFTY SGIRTNGATS ACRRSGSSFY AEMKWLLSNT DNAAFPQMTK SYKNTRKSPA LIVWGIHHSV STAEQTKLYG SGNKLVTVGS SNYQQSFVPS PGARPQVNGL SGRIDFHWLM LNPNDTVTFS FNGAFIAPDR ASFLRGKSMG IQSGVQVDAN CEGDCYHSGG TIISNLPFQN IDSRAVGKCP RYVKQRSLLL ATGMKNVPEI PKGRHHHHHH
Product Science Overview
Hemagglutinin (HA) is a surface glycoprotein found on the influenza virus, playing a crucial role in the virus’s ability to infect host cells. The H7N9 strain of the influenza A virus, particularly the Anhui 2013 variant, has garnered significant attention due to its potential to cause severe respiratory illness in humans.
The H7N9 influenza virus was first identified in humans in China in 2013. The Anhui 2013 strain, specifically, is a reassortant virus, meaning it contains genetic material from multiple influenza viruses. Phylogenetic analysis suggests that the HA gene of this virus is derived from an H7N3 virus found in Eurasian wild birds, while the neuraminidase (NA) gene comes from either H11N9 or H2N9 viruses from wild birds. The internal genes (PB2, PB1, PA, NP, M, and NS) are from H9N2 viruses in poultry in China .
Hemagglutinin is responsible for binding the virus to the host cell’s surface receptors, facilitating viral entry. The HA protein of the H7N9 virus has undergone several mutations that enhance its ability to bind to human cell receptors, increasing its virulence. Notably, mutations such as A135T and N171S in the HA protein have been identified in the Anhui 2013 strain .
Antigenic drift refers to the gradual accumulation of mutations in the HA protein, leading to changes in its antigenic properties. This process allows the virus to evade the host’s immune response. Studies have shown that the H7N9 virus has undergone significant antigenic drift since its emergence, with mutations like A143V, A143T, and R148K reducing the virus’s susceptibility to neutralization by antisera .
The HA protein of the H7N9 virus, particularly the Anhui 2013 strain, has been found to elicit weak immune responses. This low immunogenicity may be due to regulatory T cell (Treg)-stimulating epitopes in the HA protein. Efforts to develop vaccines against H7N9 have focused on engineering the HA protein to improve its immunogenicity .
