GZMK Human, Sf9

Granzyme-K is a glycosylated polypeptide chain consisting of 247 amino acids, with a molecular mass of approximately 26.9 kDa . The human recombinant form of Granzyme-K is produced in Sf9 Baculovirus cells, which allows for the expression of the protein with post-translational modifications similar to those found in humans .

The amino acid sequence of Granzyme-K includes a 6-amino acid IgG His-Tag at the C-terminus, which facilitates its purification and detection in laboratory settings . The protein is typically stored in a sterile filtered clear solution and can be kept at 4°C for short-term use or frozen at -20°C for long-term storage .

Granzyme-K exhibits tryptase-like activity, meaning it cleaves substrates after basic amino acids such as arginine or lysine . This enzymatic activity is essential for its role in inducing apoptosis in target cells. When NK cells or CTLs recognize a target cell, they release Granzyme-K along with other granzymes and perforin. Perforin forms pores in the target cell membrane, allowing granzymes to enter the cell and trigger programmed cell death.

Recombinant Granzyme-K is widely used in research to study its role in immune responses and its potential therapeutic applications. It is utilized in various assays, including SDS-PAGE and functional studies, to understand its activity and interactions with other proteins . The high purity and biological activity of recombinant Granzyme-K make it a valuable tool for scientists investigating immune mechanisms and developing new treatments for diseases involving the immune system.

For optimal stability, Granzyme-K should be stored in phosphate-buffered saline (PBS) with 20% glycerol and 1mM dithiothreitol (DTT) at a pH of 7.4 . It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity and activity. For long-term storage, adding a carrier protein such as human serum albumin (HSA) or bovine serum albumin (BSA) is recommended .