GZMH Human, sf9

Granzyme-H Human Recombinant, sf9
Cat. No.
BT24209
Source
Sf9, Baculovirus cells.
Synonyms
Granzyme H, Granzyme H (Cathepsin G-Like 2, Protein H-CCPX), Cytotoxic T-Lymphocyte Proteinase, Cathepsin G-Like 2, CTSGL2, CCP-X, CSP-C, Cytotoxic T-Lymphocyte-Associated Serine Esterase 1, Cathepsin G-Like 2, Protein H-CCPX, Cytotoxin Serine Protease-C, Cytotoxic Serine Protease C, Protein H-CCPX, EC 3.4.21.79, EC 3.4.21, EC 3.4.21, CTLA1, CGL-2 , CGL2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GZMH produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 234 amino acids (19-246 a.a.) and having a molecular mass of 26.2kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
GZMH is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Granzyme H, also referred to as GZMH, is a member of the peptidase S1 family. This enzyme plays a crucial role in the eradication of the Hepatitis B Virus (HBV). GZMH targets and cleaves the HBx protein, a vital component for HBV replication, at the Met(79) position. This action effectively disrupts the virus's ability to multiply. The significance of GZMH in HBV clearance is further emphasized by the fact that inhibiting GZMH prevents both GZNH- and lymphokine-activated killer cell-mediated HBx degradation, ultimately hindering HBV elimination. HBV variants lacking the HBx protein exhibit resistance to GzmH-mediated, as well as lymphokine-activated killer cell-mediated, viral clearance.
Description
Produced in Sf9 Baculovirus cells, GZMH is a single, glycosylated polypeptide chain with a molecular weight of 26.2kDa. It consists of 234 amino acids (19-246 a.a.). On SDS-PAGE, the molecular size will appear between 28-40kDa. The GZMH protein is expressed with a 6 amino acid His tag at the C-Terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The GZMH protein solution is provided at a concentration of 0.25mg/ml. It is formulated in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of GZMH is determined to be greater than 90% based on SDS-PAGE analysis.
Synonyms
Granzyme H, Granzyme H (Cathepsin G-Like 2, Protein H-CCPX), Cytotoxic T-Lymphocyte Proteinase, Cathepsin G-Like 2, CTSGL2, CCP-X, CSP-C, Cytotoxic T-Lymphocyte-Associated Serine Esterase 1, Cathepsin G-Like 2, Protein H-CCPX, Cytotoxin Serine Protease-C, Cytotoxic Serine Protease C, Protein H-CCPX, EC 3.4.21.79, EC 3.4.21, EC 3.4.21, CTLA1, CGL-2 , CGL2.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
EEIIGGHEAK PHSRPYMAFV QFLQEKSRKR CGGILVRKDF VLTAAHCQGS SINVTLGAHN IKEQERTQQF IPVKRPIPHP AYNPKNFSND IMLLQLERKA KWTTAVRPLR LPSSKAQVKP GQLCSVAGWG YVSMSTLATT LQEVLLTVQK DCQCERLFHG NYSRATEICV GDPKKTQTGF KGDSGGPLVC KDVAQGILSY GNKKGTPPGV YIKVSHFLPW IKRTMKRLHH HHHH.

Product Science Overview

Introduction to Granzymes

Granzymes are a family of serine proteases that play a crucial role in the immune system. They are primarily found in the granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. These enzymes are released during the immune response to target and induce apoptosis in infected or malignant cells .

Granzyme-H: An Overview

Granzyme-H (GzmH) is one of the five human granzymes, alongside Granzyme A, B, K, and M. It is a chymotrypsin-like serine protease predominantly expressed in NK cells . GzmH has unique substrate specificity and plays a significant role in the innate immune response against tumors and viral infections .

Recombinant Granzyme-H Production

The recombinant form of Granzyme-H, produced in sf9 insect cells, is utilized for research and therapeutic purposes. The sf9 cell line, derived from the fall armyworm (Spodoptera frugiperda), is commonly used in biotechnology for the production of recombinant proteins. This system allows for the expression of human proteins with post-translational modifications similar to those in mammalian cells .

Structural Insights

The structure of Granzyme-H has been studied to understand its substrate specificity and mechanism of action. The enzyme contains a unique RKR motif that helps define its binding regions, indicating a preference for acidic residues at specific sites . This structural information is crucial for designing inhibitors that can selectively block the enzymatic and cytotoxic activity of GzmH .

Biological Functions

Granzyme-H is involved in the immune defense against transformed and virus-infected cells. It induces apoptosis through various pathways, contributing to the elimination of harmful cells. Recent studies have also highlighted its role in extracellular matrix alteration and tissue homeostasis .

Therapeutic Potential

Given its role in immune responses, Granzyme-H has potential therapeutic applications. Understanding its structure and function can aid in the development of targeted therapies for cancer and viral infections. Additionally, recombinant Granzyme-H can be used in research to further explore its biological activities and potential clinical applications .

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THE BIOTEK
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