GZMB Human

Granzyme-B Human Recombinant
Cat. No.
BT23861
Source
E.coli.
Synonyms
Granzyme B, C11, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, HLP, SECT, T-cell serine protease 1-3E, CGL1, CSPB, CTLA1, GRB, GZMB, CCPI, CGL-1, CSP-B.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GZMB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 248 amino acids (21-247 a.a.) and having a molecular mass of 27.8kDa.
GZMB is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Granzyme-B (GZMB) is a crucial component of the cell-mediated immune response, playing a vital role in target cell destruction. It is associated with a cascade of caspase activation that ultimately leads to apoptosis (programmed cell death). GZMB specifically cleaves and activates caspases 3, 7, 9, and 10, which are enzymes directly involved in executing apoptosis.
Description
Recombinant human GZMB, produced in E. coli, is a single polypeptide chain lacking glycosylation. It comprises 248 amino acids (specifically residues 21 to 247) and has a molecular weight of 27.8 kDa. This protein is engineered with a 20 amino acid His-tag at the N-terminus to facilitate purification, which is achieved using proprietary chromatographic methods.
Physical Appearance
A clear, sterile solution.
Formulation
The GZMB protein is provided in a solution at a concentration of 1 mg/ml. The solution also contains 20 mM Tris-HCl buffer at pH 8.0 and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For longer storage, freezing at -20°C is recommended. Adding a carrier protein such as HSA or BSA (at a concentration of 0.1%) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the GZMB protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Granzyme B, C11, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, HLP, SECT, T-cell serine protease 1-3E, CGL1, CSPB, CTLA1, GRB, GZMB, CCPI, CGL-1, CSP-B.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MIIGGHEAKP HSRPYMAYLM IWDQKSLKRC GGFLIQDDFV LTAAHCWGSS INVTLGAHNI KEQEPTQQFI PVKRPIPHPA YNPKNFSNDI MLLQLERKAK RTRAVQPLRL PSNKAQVKPG QTCSVAGWGQ TAPLGKHSHT LQEVKMTVQE DRKCESDLRH YYDSTIELCV GDPEIKKTSF KGDSGGPLVC NKVAQGIVSY GRNNGMPPRA CTKVSSFVHW IKKTMKRY.

Product Science Overview

Introduction

Granzyme B is a serine protease enzyme that plays a crucial role in the immune system, particularly in the process of inducing apoptosis in target cells. It is predominantly found in the granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells . The recombinant form of Granzyme B, produced through genetic engineering techniques, is used extensively in research and therapeutic applications.

Molecular Structure and Properties

Granzyme B is initially synthesized as an inactive precursor, known as a zymogen, which includes an additional amino-terminal peptide sequence. This sequence is cleaved by cathepsin C, resulting in the activation of the enzyme . The mature form of Granzyme B consists of 235 amino acids, including an 8 amino acid C-terminal His-tag, and has a molecular weight of approximately 26.6 kDa .

Due to glycosylation, the protein migrates to an approximate molecular weight of 30-40 kDa under reducing and non-reducing conditions . The enzyme is highly purified, with a purity of ≥ 95% as determined by SDS-PAGE gel and HPLC analyses .

Biological Function

Granzyme B is a key player in the immune response, particularly in the induction of apoptosis in target cells. It is released by CTLs and NK cells upon recognition of infected or malignant cells. Granzyme B enters the target cells through perforin-formed pores and initiates a cascade of proteolytic events that lead to programmed cell death .

The enzyme cleaves various substrates, including caspases and other cellular proteins, to trigger apoptosis. This process is essential for the elimination of virus-infected cells, tumor cells, and other abnormal cells from the body .

Applications in Research and Medicine

Recombinant Granzyme B is widely used in research to study the mechanisms of apoptosis and immune responses. It is also utilized in the development of therapeutic strategies for cancer and viral infections. The recombinant form allows for consistent and controlled studies, providing valuable insights into the enzyme’s function and potential applications .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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