GTF3C6 Human

General Transcription Factor IIIC Polypeptide 6 Human Recombinant
Cat. No.
BT11116
Source
Escherichia Coli.
Synonyms
bA397G5.3, C6orf51, TFIIIC35, General transcription factor 3C polypeptide 6, Transcription factor IIIC 35 kDa subunit, TFIIIC 35 kDa subunit, TFIIIC35, Transcription factor IIIC subunit 6, CDA020, NPD020.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GTF3C6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 236 amino acids (1-213 a.a) and having a molecular mass of 26.4kDa.
GTF3C6 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
General transcription factors (GTFs) are essential for RNA synthesis initiation, as RNA polymerases cannot perform this function alone. GTFs assemble into a complex at the DNA promoter region, where they recruit RNA polymerase. The GTF3C family proteins are crucial for RNA polymerase III in the production of small nuclear and cytoplasmic RNAs. These include 5S RNA, tRNA, and adenovirus-associated (VA) RNA, which are derived from both cellular and viral sources.
Description
Recombinant human GTF3C6, expressed in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 26.4 kDa. It consists of 236 amino acids, with amino acids 1-213 representing the GTF3C6 sequence. A 23-amino acid His-tag is fused to the N-terminus to facilitate purification, which is performed using proprietary chromatographic techniques.
Physical Appearance
A clear solution, sterilized by filtration.
Formulation
The GTF3C6 protein is supplied in a solution with a concentration of 0.5 mg/ml. The solution contains PBS buffer at a pH of 7.4, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. It's important to avoid repeated freezing and thawing of the product.
Purity
The purity of the GTF3C6 protein is determined to be greater than 85% using SDS-PAGE analysis.
Synonyms
bA397G5.3, C6orf51, TFIIIC35, General transcription factor 3C polypeptide 6, Transcription factor IIIC 35 kDa subunit, TFIIIC 35 kDa subunit, TFIIIC35, Transcription factor IIIC subunit 6, CDA020, NPD020.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAAADE RSPEDGEDEE EEEQLVLVEL SGIIDSDFLS KCENKCKVLG IDTERPILQV DSCVFAGEYE DTLGTCVIFE ENVEHADTEG NNKTVLKYKC HTMKKLSMTR TLLTEKKEGE ENIGGVEWLQ IKDNDFSYRP NMICNFLHEN EDEEVVASAP DKSLELEEEE IQMNDSSNLS CEQEKPMHLE IEDSGPLIDI PSETEGSVFM ETQMLP.

Product Science Overview

Introduction

General Transcription Factor IIIC Polypeptide 6 (GTF3C6) is a crucial component of the transcription machinery in eukaryotic cells. It is a subunit of the larger General Transcription Factor IIIC (TFIIIC) complex, which is essential for the transcription of genes by RNA Polymerase III. This article delves into the structure, function, and significance of GTF3C6, particularly focusing on its human recombinant form.

Structure and Composition

GTF3C6 is a protein encoded by the GTF3C6 gene in humans. The protein consists of 213 amino acids and has a molecular weight of approximately 26 kDa . The recombinant form of GTF3C6 is often expressed in Escherichia coli and purified using conventional chromatography techniques . The protein typically includes a His tag at the N-terminus to facilitate purification and detection .

Function

GTF3C6 plays a pivotal role in the assembly and function of the TFIIIC complex. TFIIIC is responsible for recognizing and binding to specific DNA sequences known as promoter regions, which are located upstream of genes transcribed by RNA Polymerase III. These genes include those encoding tRNAs, 5S rRNA, and other small RNAs essential for cellular function .

The TFIIIC complex consists of six subunits, and GTF3C6 is one of the smaller subunits. Despite its size, GTF3C6 is critical for the stability and activity of the entire complex. It interacts with other subunits to form a functional assembly that can recruit RNA Polymerase III to the promoter regions .

Biological Significance

The proper functioning of GTF3C6 and the TFIIIC complex is vital for the transcription of genes involved in protein synthesis and other fundamental cellular processes. Dysregulation of TFIIIC components, including GTF3C6, can lead to various diseases and disorders. For instance, mutations or alterations in the GTF3C6 gene have been associated with certain types of cancer, such as pancreatic intraductal papillary-colloid carcinoma .

Recombinant GTF3C6

Recombinant GTF3C6 is widely used in research to study the structure and function of the TFIIIC complex. The recombinant protein is typically produced in E. coli and purified to high purity levels (>85%) for use in various biochemical assays . Researchers utilize recombinant GTF3C6 to investigate its interactions with other TFIIIC subunits, its role in transcription initiation, and its potential as a therapeutic target.

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