GTF2F2 Human

GTF2F2, also known as RAP30, is a 30 kDa protein that plays a pivotal role in the transcription initiation process. It forms a heteromeric complex with GTF2F1, and this complex is essential for the proper functioning of RNA polymerase II . RNA polymerase II is responsible for transcribing mRNA from DNA, a critical step in gene expression.

The primary function of TFIIF is to stabilize the pre-initiation complex (PIC) and facilitate the recruitment of RNA polymerase II to the promoter regions of genes . TFIIF also assists in the transition from transcription initiation to elongation, ensuring that RNA polymerase II can efficiently transcribe the DNA template into RNA .

TFIIF interacts with several other general transcription factors, including TFIIB and TFIIE, to form a stable PIC . This complex binds to the promoter region of a gene, where TFIIF helps to position RNA polymerase II correctly. Once the PIC is assembled, TFIIF aids in the unwinding of DNA, allowing RNA polymerase II to access the template strand and begin RNA synthesis .

Additionally, TFIIF has been shown to have helicase activity, which helps in the unwinding of DNA during transcription . This activity is crucial for the progression of the transcription machinery along the DNA template.

Recombinant DNA technology has enabled the production of human GTF2F2 in various expression systems . This recombinant protein is used in research to study the detailed mechanisms of transcription initiation and regulation. By using recombinant GTF2F2, scientists can investigate its interactions with other transcription factors and its role in gene expression more precisely .

Understanding the function and regulation of GTF2F2 is vital for comprehending how genes are expressed and regulated in human cells. Dysregulation of transcription factors like GTF2F2 can lead to various diseases, including cancer . Therefore, studying GTF2F2 and its interactions provides insights into potential therapeutic targets for treating such conditions .