GSTT2 Human

Glutathione S-Transferase Theta-2 Human Recombinant
Cat. No.
BT8302
Source
E.coli.
Synonyms
Glutathione S-transferase theta 2, GST class-theta-2, GSTT2B, Glutathione S-transferase theta-2B, EC 2.5.1.18.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GSTT2 Recombinant produced in E. coli is a single polypeptide chain containing 264 amino acids (1-244) and having a molecular mass of 29.6kDa.
GSTT2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
GSTT2 is a member of the glutathione s-transferase (GST) family. Eight GST protein families exist: alpha, kappa, mu, omega, pi, sigma, theta, and zeta. Each GST family contains several proteins with distinct cellular functions. GSTT1 and GSTT2, members of the theta family, share 55% amino acid sequence homology and play a crucial role in human carcinogenesis. The theta genes share a similar structure, each composed of five exons with identical exon/intron boundaries.
Description
Recombinant GSTT2, produced in E. coli, is a single polypeptide chain consisting of 264 amino acids (residues 1-244) with a molecular weight of 29.6 kDa. A 20 amino acid His-tag is fused to the N-terminus of GSTT2. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The GSTT2 solution (1 mg/ml) is supplied in 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1 mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), store the solution at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 95% by SDS-PAGE analysis.
Synonyms
Glutathione S-transferase theta 2, GST class-theta-2, GSTT2B, Glutathione S-transferase theta-2B, EC 2.5.1.18.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGLELFLDLV SQPSRAVYIF AKKNGIPLEL RTVDLVKGQH KSKEFLQINS LGKLPTLKDG DFILTESSAI LIYLSCKYQT PDHWYPSDLQ ARARVHEYLG WHADCIRGTF GIPLWVQVLG PLIGVQVPEE KVERNRTAMD QALQWLEDKF LGDRPFLAGQ QVTLADLMAL EELMQPVALG YELFEGRPRL AAWRGRVEAF LGAELCQEAH SIILSILEQA AKKTLPTPSP EAYQAMLLRI ARIP

Product Science Overview

Introduction

Glutathione S-Transferase Theta-2 (GSTT2) is an enzyme encoded by the GSTT2 gene in humans. This enzyme belongs to the glutathione S-transferase (GST) superfamily, which plays a crucial role in the detoxification of endogenous and exogenous compounds by catalyzing the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds .

Structure and Classification

GSTs are divided into several classes, including alpha, mu, pi, theta, and zeta. GSTT2 is a member of the theta class, which also includes GSTT1 and GSTT2B. These enzymes share structural similarities but have distinct functional roles. GSTT2 and GSTT2B are nearly identical, sharing 55% amino acid identity with GSTT1 .

Function

The primary function of GSTT2 is to facilitate the conjugation of reduced glutathione to various substrates, aiding in the detoxification process. This activity is essential for protecting cells from oxidative stress and maintaining cellular homeostasis. GSTT2 has also been shown to possess sulfatase activity, further contributing to its role in detoxification .

Genetic and Molecular Aspects

The GSTT2 gene is located on human chromosome 22q11.2. Interestingly, the GSTT2 gene is a pseudogene in some populations, meaning it may not be functional in those individuals. This variation can have implications for susceptibility to certain diseases and responses to environmental toxins .

Clinical Significance

GSTT2 has been implicated in various diseases, including certain types of cancer. The enzyme’s role in detoxification suggests that variations in GSTT2 activity could influence an individual’s risk of developing cancer and other diseases related to oxidative stress. Additionally, GSTT2 is involved in pathways related to biotransformation and glutathione conjugation, highlighting its importance in cellular metabolism .

Applications

Recombinant human GSTT2 is used in research to study its enzymatic activity and potential therapeutic applications. By understanding the structure and function of GSTT2, scientists can develop strategies to modulate its activity, potentially leading to new treatments for diseases associated with oxidative stress and detoxification .

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