GST Monoclonal antibody

Glutathione-S-Transferase, Mouse antibody
Cat. No.
BT5315
Source
Synonyms
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Purity
Usage
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Description

Monoclonal antibodies are produced by immunizing mouse with GST protein.

Product Specs

Introduction
The GST family of enzymes consists of cytosolic, mitochondrial, and microsomal proteins with molecular weights ranging from 45 to 55 kDa (dimer). These enzymes are involved in various reactions, utilizing a wide range of endogenous and xenobiotic substrates. GSTs catalyze the conjugation of reduced glutathione's sulfhydryl group to electrophilic centers on diverse substrates. This activity is crucial for detoxifying endogenous compounds like oxidized lipids and metabolizing xenobiotics. Additionally, GSTs bind to toxins and act as transport proteins. GSTs play a significant role in the 'GST gene fusion system,' facilitating protein purification and detection. In this system, a GST sequence is incorporated into an expression vector alongside the gene of interest. The resulting fusion protein, containing the protein of interest linked to GST, is expressed upon induction. This GST-fusion protein can be readily purified using glutathione's high affinity for GST. Fusion proteins are valuable tools for studying direct protein-protein interactions. The GST tag, consisting of 220 amino acids, is relatively large compared to other tags like myc or FLAG. It is typically fused to the protein's N-terminus. Commercially available GST-tagged plasmids often include a thrombin domain, allowing for the removal of the GST tag during purification. The GST tag is frequently employed to isolate and purify proteins containing the GST-fusion. These fusion proteins can be produced in Escherichia coli as recombinant proteins.
Description
Monoclonal antibodies are generated by immunizing mice with purified GST protein.
Formulation
1×PBS with 50% glycerol.
Titer
Western Blotting: 1 µg/ml.
Applications
Western Blot, Immunoprecipitation.
Type
Mouse Antibody Monoclonal.
Clone
PGSTSHG.
Ig Subclass
Mouse IgG2b.

Product Science Overview

Introduction

Glutathione-S-Transferase (GST) is a crucial enzyme involved in the detoxification processes within cells. It catalyzes the conjugation of the reduced form of glutathione (GSH) to electrophilic substrates, aiding in the neutralization of toxic compounds. The GST enzyme is widely used in molecular biology and biochemistry, particularly in the form of GST fusion proteins for various applications.

GST Mouse Antibody

The GST mouse antibody is a monoclonal antibody produced in mice, specifically designed to target and bind to the GST enzyme. This antibody is derived from hybridoma cells, which are created by fusing mouse myeloma cells with splenocytes from BALB/c mice immunized with a purified recombinant GST fusion protein .

Characteristics
  • Biological Source: Mouse
  • Isotype: IgG2b
  • Form: Purified immunoglobulin in a buffered aqueous solution
  • Concentration: Approximately 1 mg/mL
  • Storage Temperature: -20°C
  • Applications: Suitable for ELISA, dot blot, immunoblotting, immunofluorescence, and immunoprecipitation .
Applications in Research

The GST mouse antibody is extensively used in various research applications:

  1. Immunoblotting: Detecting GST-tagged proteins in Western blot assays.
  2. Immunoprecipitation: Isolating GST fusion proteins from cell lysates.
  3. ELISA: Quantifying GST-tagged proteins in samples.
  4. Immunofluorescence: Visualizing the localization of GST-tagged proteins within cells .
Significance in Molecular Biology

GST fusion proteins are widely used in molecular biology for studying protein-protein and protein-DNA interactions. The GST tag facilitates the purification and detection of recombinant proteins, making it a valuable tool in biochemical research. The GST mouse antibody specifically binds to the GST tag, allowing researchers to isolate and analyze GST-tagged proteins efficiently .

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