GH Carp

Growth Hormone Carp Recombinant
Cat. No.
BT12196
Source
Escherichia Coli.
Synonyms
GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Growth Hormone Carp Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 188 amino acids & having a molecular mass of 21,408 Dalton.
Growth Hormone Carp is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Growth Hormone (GH) is part of the somatotropin/prolactin hormone family, crucial for growth regulation. The GH gene, along with four related genes, resides on chromosome 17 at the growth hormone locus. Their arrangement, thought to be a result of gene duplications, shows them in the same transcriptional orientation. These five genes share significant sequence similarity. Adding complexity, alternative splicing creates additional isoforms of each growth hormone, increasing diversity and potential specialization. While expressed in the pituitary gland, this specific family member is not found in placental tissue, unlike the other four genes at the growth hormone locus. Mutations or deletions within this gene can lead to growth hormone deficiency and consequently, short stature.
Description
Recombinant Carp Growth Hormone, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 188 amino acids, resulting in a molecular weight of 21,408 Daltons. The purification of Carp Growth Hormone is achieved using proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The Carp GH was lyophilized from a concentrated (1mg/ml) solution with 0.3% NaHCO3, adjusted to a pH of 8.
Solubility
For reconstitution of the lyophilized Carp Growth Hormone, it is recommended to use sterile 18MΩ-cm H2O at a concentration not less than 100µg/ml. This solution can be further diluted into other aqueous solutions as needed.
Stability
Lyophilized Carp Growth Hormone remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, Carp Growth Hormone should be stored at 4°C for 2-7 days. For future use, store below -18°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0% as determined by: (a) Size Exclusion Chromatography-High Performance Liquid Chromatography (SEC-HPLC) analysis. (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
Carp GH exhibits biological activity in rat 3T3 F442A preadipocytes. However, it is 15-fold less potent compared to bovine GH. Conversely, it demonstrates equal potency in vivo for promoting carp growth (Fine et al., 1993). Additionally, carp GH forms a 1:2 complex with the extracellular domain of the ovine growth hormone receptor.
Synonyms
GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and found to be Ser-Asp-Asn-Gln-Arg.

Product Science Overview

Introduction

Growth hormone carp recombinant refers to the genetically engineered growth hormone specifically designed for carp. This recombinant hormone is produced using advanced biotechnological techniques to enhance the growth rate and overall productivity of carp, a widely farmed fish species. The development of recombinant growth hormones for aquaculture aims to improve feed efficiency, growth performance, and overall sustainability of fish farming practices.

Development and Preparation

The development of recombinant growth hormone for carp involves the insertion of the growth hormone gene from carp into a suitable host organism, typically bacteria or yeast, which then produces the hormone in large quantities. The process includes several key steps:

  1. Gene Isolation: The growth hormone gene is isolated from carp using molecular biology techniques.
  2. Vector Construction: The isolated gene is inserted into a plasmid vector, which is a small circular DNA molecule used to transfer genetic material.
  3. Transformation: The plasmid vector containing the growth hormone gene is introduced into a host organism, such as Escherichia coli (E. coli) or yeast, through a process called transformation.
  4. Expression and Purification: The host organism expresses the growth hormone, which is then harvested and purified using various biochemical techniques to obtain the recombinant growth hormone.
Mechanism of Action

The recombinant growth hormone functions similarly to the natural growth hormone produced by carp. It binds to specific receptors on the surface of target cells, triggering a cascade of biochemical reactions that promote growth and development. The hormone stimulates protein synthesis, increases cell division, and enhances the utilization of nutrients, leading to improved growth rates and feed conversion efficiency in carp.

Applications in Aquaculture

The use of recombinant growth hormone in carp farming has several potential benefits:

  1. Enhanced Growth Rates: Carp treated with recombinant growth hormone exhibit significantly faster growth rates compared to untreated fish.
  2. Improved Feed Efficiency: The hormone enhances the ability of carp to convert feed into body mass, reducing the overall feed costs for farmers.
  3. Sustainability: By improving growth performance and feed efficiency, the use of recombinant growth hormone can contribute to more sustainable aquaculture practices, reducing the environmental impact of fish farming.
Safety and Regulatory Considerations

The use of recombinant growth hormone in aquaculture is subject to rigorous safety and regulatory assessments to ensure its safety for both the fish and consumers. Studies have shown that the recombinant growth hormone used in carp does not pose any significant risks to human health when consumed as part of the fish. Regulatory agencies evaluate the potential environmental impact, food safety, and ethical considerations before approving the use of recombinant growth hormones in aquaculture.

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