Toxoplasma P35

Toxoplasma gondii has a two-phase life cycle. The sexual phase occurs solely in felines, making them the primary host. The asexual phase can transpire in any warm-blooded creature, including mammals and birds. T. gondii constructs daughter scaffolds within its host's cells. In intermediate hosts, it forms intracellular parasitophorous vacuoles containing slowly replicating bradyzoites. These vacuoles, primarily found in muscles and the brain, evade the host's immune system. While antibiotic resistance varies, complete eradication of these cysts is challenging. Within these vacuoles, T. gondii multiplies through binary fission until the host cell ruptures, releasing tachyzoites, the motile, asexually reproducing form. Unlike bradyzoites, tachyzoites are usually effectively neutralized by the host's immune response. However, some may infect new cells and transform into bradyzoites, perpetuating the infection. Recombinant Toxoplasma Gondii P35 (GRA8) serves as an antigen to detect specific antibodies for diagnosing Toxoplasma gondii infection.

Recombinant Toxoplasma Gondii P35 (GRA8), encompassing 217 amino acids, was purified from E. coli. This protein is fused to a GST tag at its N-terminus and purified using a proprietary chromatographic method.

Sterile Filtered clear solution.

The Toxoplasma P35 solution consists of 0.5mM EDTA, Phosphate buffered saline, with a pH of 7.4.

For optimal storage, keep at 4°C if using the entire vial within 2-4 weeks. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. It is crucial to avoid repeated freezing and thawing.

The protein purity exceeds 95%, as determined by 12% PAGE (coomassie staining).

This product is suitable for immunoassays.

Dense granule protein GRA8, GRA8, TGME49_254720

Escherichia Coli.