GPC4 Human

Glypican-4 Human Recombinant
Cat. No.
BT12227
Source
Escherichia Coli.
Synonyms
Glypican 4, Glypican Proteoglycan 4, K-glypican, DJ900E8.1 (Glypican 4), glypican-4.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GPC4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids (401-529a.a) and having a molecular mass of 18kDa.
GPC4 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Glypican 4 (GPC4) belongs to the family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) involved in regulating cell division and growth. GPC4 is widely distributed in human tissues, such as the lungs, kidneys, heart, placenta, skeletal muscle, and pancreas. It is also found in astrocytes, haematopoietic-progenitor cells, and bone-marrow-stromal cells.
Description
Recombinant Human GPC4, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 165 amino acids (401-529a.a). It has a molecular weight of 18 kDa. This GPC4 protein is fused to a 36 amino acid His-tag at its N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The GPC4 protein solution is provided at a concentration of 0.25 mg/ml. It is formulated in 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advised for extended storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the GPC4 protein is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
Glypican 4, Glypican Proteoglycan 4, K-glypican, DJ900E8.1 (Glypican 4), glypican-4.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSSSLP SNVCNDERMA AGNGNEDDCW NGKGKSRYLF AVTGNGLANQ GNNPEVQVDT SKPDILILRQ IMALRVMTSK MKNAYNGNDV DFFDISDESS GEGSGSGCEY QQCPSEFDYN ATDHAGKSAN EKADS

Product Science Overview

Introduction

Glypican-4 is a member of the glypican family, which consists of heparan sulfate proteoglycans that are attached to the cell surface via a glycosylphosphatidylinositol (GPI) anchor. These proteoglycans play crucial roles in cell growth, differentiation, and morphogenesis. Glypican-4, in particular, is encoded by the GPC4 gene in humans and is involved in various biological processes, including the development of the central nervous system and kidney tubules .

Structure and Expression

Glypican-4 is characterized by a core protein that is approximately 60 kDa in size, which is substituted with heparan sulfate chains. The recombinant form of human Glypican-4 is often produced in systems such as Escherichia coli or Chinese Hamster Ovary (CHO) cells. The recombinant protein typically includes a fragment of the human Glypican-4 protein, ranging from amino acids 401 to 529 .

Biological Functions

Glypican-4 is involved in several key biological functions:

  • Cell Signaling: Glypican-4 interacts with various growth factors, including fibroblast growth factors (FGFs), and modulates their signaling pathways. This interaction is crucial for processes such as cell proliferation and differentiation .
  • Development: It plays a significant role in the development of the central nervous system and kidney tubules. The presence of Glypican-4 is essential for proper morphogenesis and organogenesis .
  • Metabolism: Glypican-4 is also expressed in adipose tissue and has been implicated in the regulation of metabolic processes .
Clinical Relevance

Mutations or dysregulation of the GPC4 gene have been associated with various diseases, including Keipert Syndrome and Simpson-Golabi-Behmel Syndrome, Type 1 . These conditions are characterized by developmental abnormalities and overgrowth syndromes, highlighting the importance of Glypican-4 in normal development and growth regulation.

Applications

Recombinant human Glypican-4 is used in various research applications, including:

  • High-Throughput Screening: It is utilized in assays to study protein-protein interactions and to screen for potential therapeutic compounds .
  • Functional Studies: Researchers use recombinant Glypican-4 to investigate its role in cell signaling pathways and its interactions with other proteins .
  • Disease Modeling: It is employed in the study of diseases associated with GPC4 mutations to understand the underlying mechanisms and to develop potential treatments .

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