GPC4 Antibody

Glypican-4 (GPC4) is a member of the glypican family of heparan sulfate proteoglycans, which are anchored to the cell surface via a glycosylphosphatidylinositol (GPI) anchor. These proteoglycans play crucial roles in cell growth, differentiation, and morphogenesis. Glypican-4 is also known as K-Glypican and has a protein core of approximately 60 kDa, with the entire proteoglycan weighing around 220 kDa due to the attached heparan sulfate chains .

Glypican-4 is expressed in various tissues, including the brain, kidney, adrenal gland, and fat tissue . It is particularly significant in the developing brain, where it is found in lateral ventricles surrounding the telencephalon, the dentate gyrus, proliferating neuroepithelial cells, and neural precursors . Glypican-4 binds to basic fibroblast growth factor (FGF), which is essential for various cellular processes .

In the developing brain, Glypican-4 inhibits the dopaminergic differentiation of neurons . This function is crucial for the proper development of neural circuits and the maintenance of neural stem cells. The expression pattern of Glypican-4 in the brain suggests its involvement in neurogenesis and the formation of neural networks .

The Mouse Anti-Human Glypican-4 antibody is a monoclonal antibody that specifically detects human Glypican-4 in various assays, including ELISA, flow cytometry, and Western blot . This antibody is produced by immunizing mice with a recombinant human Glypican-4 protein expressed in Chinese hamster ovary (CHO) cells . The antibody is purified using protein A or G from hybridoma culture supernatant .

The Mouse Anti-Human Glypican-4 antibody is used in various research applications to study the expression and function of Glypican-4. It is particularly useful in assays that require the detection of Glypican-4 in human tissues and cells . The antibody can be used as a capture or detection antibody in ELISA, as well as for staining membrane-associated proteins in flow cytometry .