Gliadin Gamma 18.6kDa Wheat
Greater than 95% as determined by SDS-PAGE.
Description
Gliadin is a cDNA coding for an epitope-carrying fragment of a wheat gamma-gliadin isoform, having a molecular mass of 19 kDa (high proline content and the low pI are likely causes for the observed discrepancy between calculated molecular weight and the observed electrophoretic mobility of approx. 50 kDa on standard SDS-PAGE), pH 4.6. By sequence design the epitopes correspond to the deamidated neo-epitopes, which in the natural antigen are formed by transglutaminase-mediated glutamine side chain deamidation. Gliadin protein is fused to a hexa-histidine purification tag.
Product Specs
Gliadin, encoded by a cDNA, represents an epitope-carrying fragment of a wheat gamma-gliadin isoform. It possesses a molecular mass of 19 kDa (the discrepancy between the calculated molecular weight and the observed electrophoretic mobility of approximately 50 kDa on standard SDS-PAGE is likely attributed to its high proline content and low pI) and a pH of 4.6. The epitopes are designed to correspond to the deamidated neo-epitopes, which are generated in the natural antigen through transglutaminase-mediated deamidation of glutamine side chains. The gliadin protein is fused to a hexa-histidine purification tag.
For short-term storage (2-4 weeks), maintain at 4°C.
For long-term storage, freeze at -20°C.
Minimize repeated freeze-thaw cycles.
1. Binds to human IgG and IgA autoantibodies associated with celiac disease.
2. Suitable for standard ELISA tests (checkerboard analysis of positive and negative sera panels) and immunodot assays.
Purity exceeds 95% as determined by SDS-PAGE analysis.
Product Science Overview
Gliadin Gamma 18.6kD Wheat Recombinant is a cDNA coding for an epitope-carrying fragment of a wheat gamma-gliadin isoform. It has a molecular mass of approximately 18,616 Daltons . The high proline content and low isoelectric point (pI) of gliadins contribute to the observed discrepancy between the calculated molecular weight and the electrophoretic mobility observed on standard SDS-PAGE, which is around 47-50 kDa .
This recombinant protein is typically expressed in Escherichia coli (E. coli) and is purified using a hexa-histidine purification tag . The protein is supplied in a sterile filtered clear solution, formulated in 20mM HEPES buffer (pH 8.0), 200mM NaCl, and 20% glycerol . The purity of the protein is greater than 94-95% as determined by SDS-PAGE .
Gliadin Gamma 18.6kD Wheat Recombinant is particularly significant in the context of celiac disease. Celiac disease is a chronic gastrointestinal disorder characterized by an abnormal immune response to gluten. The disease leads to the flattening of the jejunal mucosa and intestinal lesions of varying severity in genetically predisposed individuals .
The recombinant gliadin protein contains deamidated neo-epitopes, which are formed by transglutaminase-mediated glutamine side chain deamidation in the natural antigen . These epitopes are crucial for the binding of IgG- and IgA-type human autoantibodies associated with celiac disease .
Gliadin Gamma 18.6kD Wheat Recombinant is used in various immunological assays, including:
