Gliadin Gamma Wheat

Gliadin Gamma Wheat Recombinant
Cat. No.
BT11557
Source
Escherichia Coli.
Synonyms
Appearance
Sterile Filtered clear solution.
Purity
Protein is >90% pure.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Recombinant Wheat Gliadin Gamma protein produced in E.Coli and fused to a 6 His Tag at C-terminus, having a theoretical Mw of 37945.14 Dalton, pI 7.70.
Purified by proprietary chromatographic technique.

Product Specs

Introduction
Celiac disease, a chronic gastrointestinal disorder, can be triggered in genetically predisposed individuals by wheat gliadin and similar gluten proteins found in barley, rye, and potentially oats. This immune response results in the flattening of the jejunal mucosa and varying degrees of intestinal lesions. While not a typical autoimmune condition, Celiac disease is linked to anti-tissue transglutaminase antibodies. Gliadin antibody tests are highly recommended for screening at-risk populations for CD and other gluten sensitivities. Historically, serological tests for gliadin antibodies lacked precision for accurate diagnosis due to the absence of deamidated epitopes in the traditional gliadin fraction used in test kits. THE BioTek's deamidated Gliadin isoform addresses this by matching the deamidated neo-epitopes, which are naturally formed in the antigen through transglutaminase-mediated glutamine deamidation.
Description
This product consists of recombinant Wheat Gliadin Gamma protein. It is produced in E.Coli with a C-terminal 6 His Tag fusion. This protein has a theoretical molecular weight of 37945.14 Dalton and an isoelectric point (pI) of 7.70. Purification is achieved through a proprietary chromatographic method.
Physical Appearance
The product is a clear solution that has been sterilized by filtration.
Formulation
The Gliadin Gamma protein is provided in a solution at a concentration of 1mg/ml. The solution buffer is 10mM Tris-HCl with a pH of 7.2.
Stability
For optimal stability, Gliadin Gamma should be stored at temperatures below -18°C. While it can remain stable at 4°C for up to one week, repeated freezing and thawing cycles should be avoided.
Purity
The purity of the Gliadin Gamma protein in this product exceeds 90%.
Source
Escherichia Coli.
Amino Acid Sequence
MKTLLILTILAMAITIGTANIQVDPSGQVQWLQQQLVPQLQQPLSQQPQQTFPQPQQTFPH
QPQQQVPQPQQPQQPFLQPQQPFPQQPQQPFPQTQQPQQPFPQQPQQPFPQTQQPQQ
PFPQQPQQPFPQTQQPQQPFPQLQQPQQPFPQPQQQLPQPQQPQQSFPQQQRPFIQPSL
QQQLNCKNILLQQSKPASLVSSLWSIIWPQSDCQVMRQQCCQQLAQIPQQLQCAAIHSVVH
SIIMQQQQQQQQQQGIDIFLPLSQHEQVGQGSLVQGQGIIQPQQPAQLEAIRSLVLQTLPSM
CNVYVPPECSIMRAPFASIVAGIGGQHHHHHH.

Product Science Overview

Gliadin Gamma Protein Family

Gliadins are classified into four types: alpha, beta, gamma, and omega. Among these, gamma-gliadins are considered to be the most ancient and complex. They are encoded by a multigene family, which means there are multiple genes responsible for producing these proteins . The gamma-gliadin genes are highly diverse, with significant variations in their sequences. This diversity contributes to the different functional properties of the proteins .

Nutritional and Health Implications

Gamma-gliadins are rich in essential amino acids, making them an important nutritional component of wheat. However, they also contain epitopes that can trigger celiac disease, an autoimmune disorder where the ingestion of gluten leads to damage in the small intestine . Research has shown that certain gamma-gliadin genes are less likely to contain these toxic epitopes, suggesting the possibility of breeding wheat varieties that are safer for individuals with celiac disease .

Recombinant Gamma-Gliadins

Recombinant DNA technology has been employed to study and manipulate gamma-gliadins. By isolating and cloning specific gamma-gliadin genes, scientists can better understand their structure and function. This technology also allows for the development of wheat varieties with modified gamma-gliadin content, potentially reducing the risk of celiac disease while maintaining the desirable baking properties of wheat flour .

Future Prospects

The study of gamma-gliadins and their recombinant forms holds great promise for both agriculture and medicine. By leveraging genetic engineering, it is possible to create wheat varieties that are not only more nutritious but also safer for individuals with gluten-related disorders. This could lead to significant advancements in the production of gluten-free products and improve the quality of life for those affected by celiac disease .

In conclusion, the research and development of gliadin gamma wheat recombinant represent a significant step forward in the quest for healthier and more sustainable food sources. As our understanding of these proteins continues to grow, so too will our ability to harness their potential for the benefit of society.

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