GFRA1 Rat
Sf9, Baculovirus cells.
GDNF family receptor alpha-1, GDNF receptor alpha-1, GDNFR-alpha-1, GFR-alpha-1, RET ligand 1, TGF-beta-related neurotrophic factor receptor 1, Gfra1, Gdnfra, Retl1, Trnr1.
Greater than 85% as determined by SDS-PAGE.
Description
GFRA1 Rat Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 645 amino acids (25-430a.a.) and having a molecular mass of 72.3kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa). GFRA1 is expressed with a 239 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
GDNF family receptor alpha-1, GDNF receptor alpha-1, GDNFR-alpha-1, GFR-alpha-1, RET ligand 1, TGF-beta-related neurotrophic factor receptor 1, Gfra1, Gdnfra, Retl1, Trnr1.
Sf9, Baculovirus cells.
DRLDCVKASD QCLKEQSCST KYRTLRQCVA GKETNFSLTS GLEAKDECRS AMEALKQKSL YNCRCKRGMK KEKNCLRIYW SMYQSLQGND LLEDSPYEPV NSRLSDIFRA VPFISDVFQQ VEHISKGNNC LDAAKACNLD DTCKKYRSAY ITPCTTSMSN EVCNRRKCHK ALRQFFDKVP AKHSYGMLFC SCRDIACTER RRQTIVPVCS YEERERPNCL SLQDSCKTNY ICRSRLADFF TNCQPESRSV SNCLKENYAD CLLAYSGLIG TVMTPNYVDS SSLSVAPWCD CSNSGNDLED CLKFLNFFKD NTCLKNAIQA FGNGSDVTMW QPAPPVQTTT ATTTTAFRVK NKPLGPAGSE NEIPTHVLPP CANLQAQKLK SNVSGSTHLC LSDSDFGKDG LAGASSLEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH.
Product Science Overview
GFRα1 is a glycosylphosphatidylinositol (GPI)-anchored cell surface receptor. It primarily binds to GDNF, forming a complex that interacts with the RET tyrosine kinase receptor. This interaction triggers intracellular signaling cascades that promote neuronal survival and differentiation .
The recombinant form of GFRα1, particularly the rat version, is produced using various expression systems, including insect cells (e.g., Spodoptera frugiperda, Sf21) and mammalian cells (e.g., NS0 mouse myeloma cells). The recombinant protein is often used in research to study its binding properties, signaling mechanisms, and potential therapeutic applications .
Recombinant GFRα1 is utilized in various experimental setups, including:
- Cell Proliferation Assays: It is used to measure the proliferative effects of GDNF on specific cell lines, such as SH-SY5Y human neuroblastoma cells .
- Binding Studies: Functional ELISA assays are conducted to determine the binding affinity between GFRα1 and GDNF .
- Neurite Outgrowth Assays: These assays assess the ability of GFRα1 to confer GDNF responsiveness to neuronal cells, promoting neurite extension .
The GDNF-GFRα1-RET signaling pathway is crucial for the maintenance and repair of the nervous system. Dysregulation of this pathway has been implicated in various neurodegenerative diseases, such as Parkinson’s disease and amyotrophic lateral sclerosis (ALS). Therefore, understanding the molecular mechanisms of GFRα1 and its interactions with GDNF is vital for developing potential therapeutic strategies .
