GFRA1 Human
HEK293 cells.
GDNF receptor alpha-1, GDNFR-alpha-1, GFRalpha-1, RET ligand 1, TGF-beta-related neurotrophic factor receptor 1, GDNFRA1, RET1L2, RETL1, Glial Cell LineDerived Neurotrophic Factor Receptor Alpha, TRNR1, GPILinked Anchor Protein, PI-Linked Cell-Surface.
Greater than 95.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
GFRA1 Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a 25-423) containing 409 amino acids including a 10 a.a C-terminal His tag. The total molecular mass is 46.0kDa (calculated).
Product Specs
The GDNF family receptor alpha-1, also known as GFRA1, is a member of the GDNF receptor family. This receptor is attached to the cell surface by a glycosyl-phosphatidylinositol (GPI) anchor. GFRA1 acts as a receptor for both glial cell line-derived growth factor (GDNF) and neurturin (NTN), and it plays a crucial role in activating the RET tyrosine kinase receptor. As a potent survival factor for neurons in the central and peripheral nervous systems, GFRA1 is essential for the development of the kidneys and the enteric nervous system.
Recombinant human GFRA1, produced in HEK293 cells, is a single polypeptide chain that has undergone glycosylation. It spans amino acid residues 25 to 423 (a.a 25-423), resulting in a protein with 409 amino acids, including a 10 amino acid C-terminal His tag. The calculated molecular mass of this protein is 46.0 kDa.
The GFRA1 protein solution, at a concentration of 0.5 mg/ml in phosphate buffered saline (pH 7.5) containing 5% (w/v) trehalose, was filtered through a 0.4 μm filter before being lyophilized.
To create a working stock solution of roughly 0.5 mg/ml, it is advised to dissolve the lyophilized pellet in deionized water completely. It is important to note that GFRA1 is not sterile. Prior to using it in cell culture, the product must be filtered using a sterile filter that is suitable.
The purity of the protein is determined to be greater than 95.0% based on SDS-PAGE analysis.
GDNF receptor alpha-1, GDNFR-alpha-1, GFRalpha-1, RET ligand 1, TGF-beta-related neurotrophic factor receptor 1, GDNFRA1, RET1L2, RETL1, Glial Cell LineDerived Neurotrophic Factor Receptor Alpha, TRNR1, GPILinked Anchor Protein, PI-Linked Cell-Surface.
HEK293 cells.
DRLDCVKASD QCLKEQSCST KYRTLRQCVA GKETNFSLAS GLEAKDECRS AMEALKQKSL YNCRCKRGMK KEKNCLRIYW SMYQSLQGND LLEDSPYEPV NSRLSDIFRV VPFISVEHIP KGNNCLDAAK ACNLDDICKK YRSAYITPCT TSVSNDVCNR RKCHKALRQF FDKVPAKHSY GMLFCSCRDI ACTERRRQTI VPVCSYEERE KPNCLNLQDS CKTNYICRSR LADFFTNCQP ESRSVSSCLK ENYADCLLAY SGLIGTVMTP NYIDSSSLSV APWCDCSNSG NDLEECLKFL NFFKDNTCLK NAIQAFGNGS DVTVWQPAFP VQTTTATTTT ALRVKNKPLG PAGSENEIPT HVLPPCANLQ AQKLKSNVSG NTHLCISNGN YEKEGLGAS H HHHHHHHHH.
Product Science Overview
GDNF Family Receptor Alpha 1 (GFRA1) is a protein encoded by the GFRA1 gene in humans. It is a member of the GDNF receptor family and plays a crucial role in the nervous system by mediating the effects of neurotrophic factors such as Glial cell line-derived neurotrophic factor (GDNF) and Neurturin (NTN).
GFRA1 is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor. This means it is anchored to the cell membrane via a GPI anchor, which is a glycolipid that attaches proteins to the cell membrane. GFRA1 serves as a receptor for GDNF and NTN, facilitating their binding and subsequent activation of the RET tyrosine kinase receptor .
Mutations or dysregulation of the GFRA1 gene have been implicated in several disorders. Notably, GFRA1 is considered a candidate gene for Hirschsprung disease, a congenital condition characterized by the absence of ganglion cells in the intestines, leading to severe constipation or intestinal obstruction .
Recombinant human GFRA1 is produced using recombinant DNA technology, which involves inserting the GFRA1 gene into a suitable expression system, such as bacteria or mammalian cells, to produce the protein in large quantities. This recombinant protein is used in various research and therapeutic applications, including studies on neuronal survival, differentiation, and neurodegenerative diseases .
