GMNN Human

Geminin Human Recombinant
Cat. No.
BT12758
Source
Escherichia Coli.
Synonyms
GMNN, Geminin, DNA Replication Inhibitor, Gem, RP3-369A17.3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Geminin Human Recombinant fused to N-terminal His-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 245 amino acids and having a molecular mass of 27.7 kDa.

Product Specs

Introduction
Geminin, a nuclear protein with a molecular weight of 25 kDa, plays a crucial role in regulating DNA replication. Its levels fluctuate throughout the cell cycle, inhibiting replication during the S phase by binding to Cdt1 and undergoing degradation during mitosis. Beyond its regulatory function, Geminin participates in neural differentiation and interacts with Six3 and Hox homeodomain proteins during embryogenesis, modulating their activity. Notably, Geminin exhibits dual functionality in cell cycle control. In the S phase, it acts as a negative regulator of DNA replication, preventing multiple rounds of replication. Conversely, during mitosis (M phase), Geminin stabilizes the replication factor Cdt1, promoting DNA replication in the subsequent cell cycle. This intricate balance ensures the fidelity of DNA replication with each cell cycle. Dysregulation of Geminin is observed in various cancers and cancer cell lines, characterized by its overexpression. This upregulation highlights Geminin's potential role in driving DNA replication and cell cycle progression in malignant contexts. The protein's expression pattern reveals its absence during the G1 phase and accumulation throughout the S, G2, and M phases, followed by a decline during the metaphase/anaphase transition of mitosis.
Description
Recombinant human Geminin, with an N-terminal His-Tag, is produced in E. coli. This non-glycosylated polypeptide chain comprises 245 amino acids, resulting in a molecular weight of 27.7 kDa.
Physical Appearance
The product appears as a clear and colorless solution after sterile filtration.
Formulation
This protein solution is formulated in a buffer containing 20mM Tris (pH 8), 100mM NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the unopened vial should be stored at 4°C. For extended storage, freezing at -20°C is recommended. To ensure optimal stability during long-term storage, adding a carrier protein like HSA or BSA (0.1%) is advisable. It's crucial to avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of this product exceeds 95%, as determined by rigorous analysis using RP-HPLC and SDS-PAGE.
Synonyms
GMNN, Geminin, DNA Replication Inhibitor, Gem, RP3-369A17.3.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNPS MKQKQEEIKE NIKNSSVPRR TLKMIQPSAS GSLVGRENEL SAGLSKRKHR NDHLTSTTSS PGVIVPESSE NKNLGGVTQE SFDLMIKENP SSQYWKEVAE KRRKALYEAL KENEKLHKEI EQKDNEIARL KKENKELAEV AEHVQYMAEL IERLNGEPLD NFESLDNQEF DSEEETVEDS LVEDSEIGTC AEGTVSSSTD
AKPCI.

Product Science Overview

Structure and Function

Geminin is a protein that inhibits the initiation of DNA replication by binding to and inhibiting the activity of Cdt1, a licensing factor necessary for the formation of the pre-replicative complex (pre-RC). The pre-RC is essential for the initiation of DNA replication, and its formation is tightly regulated to prevent re-replication of the genome within a single cell cycle.

During the G1 phase of the cell cycle, Cdt1 binds to the origin recognition complex (ORC) and Cdc6, facilitating the loading of the MCM2-7 helicase complex onto DNA, which is a critical step in the formation of the pre-RC. Geminin inhibits this process by binding to Cdt1, preventing it from interacting with the ORC and Cdc6, thereby blocking the loading of the MCM2-7 complex .

Regulation of Geminin

The activity of Geminin is regulated by the cell cycle. It is present during the S, G2, and M phases, where it inhibits the reformation of the pre-RC, thus preventing re-replication. At the metaphase-anaphase transition, Geminin is targeted for degradation by the anaphase-promoting complex (APC), allowing the formation of new pre-RCs in the subsequent G1 phase .

Clinical Significance

Geminin has been studied extensively for its role in cancer biology. Overexpression of Geminin has been observed in various types of cancers, and it is thought to contribute to the uncontrolled proliferation characteristic of cancer cells. As such, Geminin is considered a potential target for cancer therapy .

Recombinant Geminin

Recombinant Geminin refers to the protein produced through recombinant DNA technology, which involves inserting the gene encoding Geminin into an expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein can be used in various research applications, including studies on DNA replication, cell cycle regulation, and cancer biology .

Research Applications

Recombinant Geminin is widely used in research to study its interactions with other proteins involved in DNA replication and cell cycle regulation. It is also used in assays to investigate the mechanisms by which Geminin inhibits the formation of the pre-RC and to screen for potential inhibitors that could be used in cancer therapy .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

GMNN Antibody
Geminin, Mouse Anti Human
Cat. No.
BT18820
Source
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.