Introduction
Geminin is a 25 kDa nuclear protein that acts as a DNA replication inhibitor and undergoes degradation during the mitotic phase of the cell cycle. Its role in replication control involves binding to Cdt1, a licensing factor. Geminin is implicated in neural differentiation and interacts directly with Six3 and Hox homeodomain proteins during embryogenesis, inhibiting their functions. Notably, Geminin exhibits a dual functionality in DNA replication depending on the cell cycle stage. During the S phase, it acts as a negative regulator of DNA replication, and its inhibition through RNAi during this phase can induce an additional round of replication in certain genomic regions. Conversely, during the M phase (mitosis), Geminin contributes to the stabilization of the replication factor Cdt1, thereby promoting DNA replication in the subsequent cell cycle. Inhibiting Geminin during mitosis, also through RNAi, leads to the destabilization of the Cdt1 protein, ultimately impairing DNA replication in the following cell cycle.
Formulation
Supplied at a concentration of 1mg/ml in PBS, pH 7.4, with 0.1% Sodium Azide.
Storage Procedures
For short-term storage (up to 1 month), store at 4°C. For extended storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The product is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This Geminin antibody has undergone validation through ELISA and Western blot analysis, ensuring its specificity and reactivity. However, due to variations in applications, titration is recommended for each experiment to achieve optimal results. For Western blot analysis, a dilution range of 1:500 to 1:1000 is suggested, with an initial dilution of 1:500 as a starting point.
Synonyms
GMNN, Geminin, DNA Replication Inhibitor, Gem, RP3-369A17.3.
Purification Method
Geminin antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Immunogen
Anti-human Geminin mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Geminin amino acids 1-209 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.
