GDNF Human
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
GDNF is purified by proprietary chromatographic techniques.
Product Specs
GDNF is a protein that plays a crucial role in the survival, differentiation, and protection of specific nerve cells. It helps these neurons develop and function correctly. GDNF works by binding to a receptor called RET, which signals within the cell to promote its survival. This protein is important for the development of the nervous system, and alterations in its gene may be linked to certain developmental disorders. Furthermore, GDNF has demonstrated the ability to enhance the survival and specialized development of a specific type of nerve cell and boost their ability to absorb substances.
Product Science Overview
GDNF is a disulfide-bonded homodimeric protein, meaning it consists of two identical subunits linked by disulfide bonds . Each subunit is approximately 15 kDa in size and contains seven conserved cysteine residues, which are essential for the formation of the protein’s stable dimeric structure . The protein signals through a multicomponent receptor system composed of the receptor tyrosine kinase RET and one of the four GFRα (α1-α4) receptors .
GDNF has been shown to promote the survival and morphological differentiation of midbrain neurons, enhance dopamine uptake, and prevent apoptosis of motor neurons . It is also involved in the development and maintenance of the central and peripheral nervous systems, renal morphogenesis, and spermatogenesis . In addition, GDNF supports the survival and differentiation of various peripheral neurons, including sympathetic, parasympathetic, sensory, and enteric neurons .
Recombinant human GDNF is produced using Escherichia coli (E. coli) expression systems, ensuring that the protein is free from animal-derived components . This is particularly important for applications where the transmission of animal viruses and contamination must be avoided. Recombinant GDNF is optimized for use in cell culture, differentiation studies, and functional assays .
Human recombinant GDNF is widely used in research to study neuronal growth, differentiation, and survival. It is also employed in various assays to analyze apoptosis and signaling pathways . In clinical research, GDNF has shown promise in improving conditions such as Parkinson’s disease by alleviating symptoms like bradykinesia, rigidity, and postural instability .
