GDNF Human, Sf9
Sf9, Baculovirus cells.
Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, ATF1, ATF2, HFB1-GDNF, HSCR3.
Greater than 90.0% as determined by SDS-PAGE.
Description
GDNF Human Recombinant produced in Baculovirus is a single glycosylated polypeptide chain containing 113amino acids (109-211 aa) and having a molecular mass of 12.8kDa.
GDNF is fused to an 10 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Glial cell-derived neurotrophic factor (GDNF) is a protein that belongs to the GDNF family of ligands. It plays a critical role in various cellular processes, including neurite outgrowth, cell differentiation, cell survival, and cell migration. GDNF promotes the survival of neurons, particularly those affected by Parkinson's disease or amyotrophic lateral sclerosis (ALS), by interacting with GFRa receptors, primarily GFRa1. Additionally, GDNF contributes to spermatogenesis, kidney development, and has been implicated in alcohol metabolism regulation.
Recombinant Human GDNF, produced in Baculovirus, is a single glycosylated polypeptide chain consisting of 113 amino acids (109-211 aa). It has a molecular weight of 12.8 kDa. The protein includes a 10 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques.
The GDNF protein solution has a concentration of 0.25 mg/ml and is supplied in a buffer containing 10% glycerol and Phosphate-Buffered Saline (pH 7.4).
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
The purity of the GDNF protein is greater than 90.0% as determined by SDS-PAGE analysis.
Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, ATF1, ATF2, HFB1-GDNF, HSCR3.
Sf9, Baculovirus cells.
ADPMRGQRGK NRGCVLTAIH LNVTDLGLGY ETKEELIFRY CSGSCDAAET TYDKILKNLS RNRRLVSDKV GQACCRPIAF DDDLSFLDDN LVYHILRKHS AKRCGCIHHH HHH
Product Science Overview
Glial-Derived Neurotrophic Factor (GDNF) is a highly conserved neurotrophic factor that plays a crucial role in the survival and differentiation of various types of neurons. The human recombinant form of GDNF, produced in Sf9 insect cells, is particularly significant in research and therapeutic applications due to its ability to mimic the natural protein’s functions.
GDNF is a small protein encoded by the GDNF gene in humans. It is known for its ability to promote the survival and morphological differentiation of dopaminergic neurons, which are critical for motor control and are affected in diseases such as Parkinson’s disease . GDNF functions by binding to the GDNF family receptor alpha (GFRα) and the RET proto-oncogene, initiating intracellular signaling pathways that prevent apoptosis and promote neuronal survival .
The human recombinant form of GDNF is produced using the Sf9 insect cell expression system. This system is advantageous for producing complex proteins with proper folding and post-translational modifications. The recombinant GDNF is typically purified using chromatographic techniques to achieve high purity levels, ensuring its efficacy in research and therapeutic applications .
Recombinant GDNF has been shown to enhance the survival and differentiation of dopaminergic neurons in culture. It also prevents apoptosis of motor neurons induced by axotomy, a process where neurons are damaged or severed . The protein’s biological activity is measured by its ability to promote the proliferation of specific cell lines, such as rat C6 cells, with an effective dose (ED50) of less than 0.1 ng/ml .
GDNF has significant therapeutic potential, particularly in the treatment of neurodegenerative diseases like Parkinson’s disease. Its ability to support neuronal survival and differentiation makes it a promising candidate for developing treatments aimed at halting or reversing neuronal damage. Additionally, GDNF is used in various research applications to study neuronal development, survival mechanisms, and neuroprotection .
The lyophilized form of recombinant GDNF is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage. Upon reconstitution, it should be stored at 4°C for short-term use and below -18°C for long-term use, with the addition of a carrier protein to prevent freeze-thaw cycles .
